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OLS Field Name OLS Field Data
Main Title Method development and preliminary applications for leptospira spirochetes in water samples /
Author M. WALKER
Other Authors
Author Title of a Work
Walker, Mark.
CORP Author Nevada Univ., Reno.; Environmental Protection Agency, Research Triangle Park, NC. National Homeland Security Research Center.
Publisher U.S. Environmental Protection Agency,
Year Published 2008
Report Number EPA/600/R-08/017
Stock Number PB2008-108874
Subjects Leptospira spirochetes. ; Decontamination (from gases, chemicals, etc.) ; Drinking water--Analysis.
Additional Subjects Leptospira ; Water sampling ; Infectious diseases ; Zoontic diseases ; Humans ; Animals ; Contamination ; Detection ; Antibodies ; Occurrence ; Recrational areas ; Watersheds ; Hawaiian Islands ; Leptospira spirochetes
Internet Access
Description Access URL
http://www.epa.gov/NHSRC/pubs/600r08017.pdf
http://www.epa.gov/NHSRC/pubs.html
https://nepis.epa.gov/Exe/ZyPDF.cgi?Dockey=P1005VD8.PDF
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB2008-108874 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 10/16/2009
Collation [30] : digital, PDF file
Abstract
Leptospirosis is an increasingly important zoonosis that infects humans and animals through contact with contaminated water. The primary challenges for carrying out water sampling for Leptospira spirochetes include isolation, concentration, and quantitative detection of small numbers of target organisms in water. The objectives of this work were to (1) develop a protocol to apply fluorescently labeled antibodies to water samples to detect virulent serovars of Leptospira, (2) develop a protocol to isolate Leptospira spirochetes from water samples, and (3) use these techniques to assess the occurrence of Leptospira in recreational waters in watersheds on Oahu and Kauai, in the Hawaiian Islands.
Notes
"EPA/600/R-08/017 "February 2008." Title taken from title screen (viewed Nov 5, 2008).
Contents Notes
Leptospirosis is an increasingly important zoonosis that infects humans and animals through contact with contaminated water. The primary challenges for carrying out water sampling for Leptospira spirochetes include isolation, concentration, and quantitative detection of small numbers of target organisms in water. The objectives of this work were to 1) develop a protocol to apply fluorescently labeled antibodies to water samples to detect virulent serovars of Leptospira, 2) develop a protocol to isolate Leptospira spirochetes from water samples, and 3) use these techniques to assess the occurrence of Leptospira in recreational waters in watersheds on Oahu and Kauai, in the Hawaiian Islands.