Record Display for the EPA National Library Catalog


Main Title A fluorescence immunoassay technique for detecting organic environimental contaminants /
Author Lukens, Herbert R. ; Williams, Colin B. ; Levison, Stuart A. ; Dandliker, Walter B. ; Muryama., Dennis
Other Authors
Author Title of a Work
Williams, Colin B.
Levison, Stuart A.
Dandliker, Walter B.
Muryama, Dennis.
CORP Author IRT Corp., San Diego, Calif.;National Environmental Research Center, Research Triangle Park, N.C.;Scripps Clinic and Research Foundation, La Jolla, Calif.
Publisher U.S. Environmental Protection Agency, Pesticides and Toxic Substances Effects Laboratory,
Year Published 1975
Report Number EPA/650-1-75-004; INTEL-RT-8098-004; EPA-68-02-1266
Stock Number PB-243 397
OCLC Number 43079317
Subjects Immunofluorescence ; Immunoassay ; Fluorescent antigen technique ; Fluorescent antibody technique
Additional Subjects Contaminants ; Benzimidazoles ; Air pollution ; Water pollution ; Environments ; Organic compounds ; Antigen antibody reactions ; Chemical compounds ; Assaying ; Immunology ; Identifying ; Pesticides ; Pollutant identification ; Pesticide residues ; Immunofluorescence
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJBD ARCHIVE EPA 650-1-75-004 Headquarters Library/Washington,DC 09/09/2013
ELBD RPS EPA 650-1-75-004 repository copy AWBERC Library/Cincinnati,OH 08/31/2020
NTIS  PB-243 397 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation xii, 63 pages ; 28 cm.
The report describes the development and successful demonstration of an immunological assay for the detection of low molecular weight organic contaminants of environmental cocern. The specific technique described is a fluorescence polarization immunoassay, the theory of which is presented. The preparation of the two required reagents, namely a fluorescent conjugate of the contaminant of interest, together with an antibody to the contaminant, is described in detail. The specific contaminant chosen for this study was 2-aminobenzimidazole (MW = 133), a metabolite of certain fungicide agents used in agriculture. The particular fluorescent moiety chosen to form the conjugate with 2-aminobenzimidazole with fluorescein. A successful demonstration of the assay has been accomplished, and a detection sensitivity in the sub-nanogram/ml range obtained.
"EPA/650-1-75-004." "May 1975." EPA contract no. 68-02-1266, ROAP No. 21AFM, program element no. 1E1078; EPA project officer: Dr. Ronald L. Baron. Includes bibliographical references (page 35).