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Main Title Evaluation of exposure markers final report /
Author Tice, Raymond R.
Other Authors
Author Title of a Work
Nauman, C. H.
CORP Author Integrated Lab. Systems, Research Triangle Park, NC.;Environmental Monitoring Systems Lab., Las Vegas, NV.;Army Biomedical Research and Development Lab., Fort Detrick, MD.
Publisher U.S. Environmental Protection Agency, Environmental Monitoring Systems Laboratory,
Year Published 1990
Report Number EPA/600/4-90/034;68-C8-0069; EPA-68-C8-0069; PB91144675
Stock Number PB91-144675
OCLC Number 31615324
Subjects Hazardous wastes--Physiological effect ; Genetic toxicology
Additional Subjects Toxicity ; Mutagenicity tests ; DNA damage ; Biological markers ; Environmental pollutants ; Acrylamides ; Trichloroethylene ; In vivo analysis ; Leukocytes ; Mice ; Superfund sites ; Chinese hamsters ; Metabolic activation ; In vitro analysis ; Carcinogenicity tests ; Hazardous wastes ; Single gel electrophoresis ; Dimethylbenzanthracenes
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJED  EPA-600/4-90-034 OCSPP Chemical Library/Washington,DC 12/09/1994
NTIS  PB91-144675 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 95 p. : ill., charts ; 28 cm.
The primary purpose of the research has been to determine the suitability of the single cell gel (SCG) assay technique for detecting DNA damage induced by genotoxic pollutants in cells sampled from various organs of rodents and in cells sampled from humans. The major chemicals evaluated include acrylamide, trichloroethylene, and dimethylbenzanthracene. The focus of the research has been on: (1) evaluating the specificity and sensitivity of the technique by determining the magnitude and kinetics of DNA damage induced in cultured mammalian cells by a variety of genotoxic chemicals; (2) developing appropriate methods for isolating individual cells from organs of rodents; (3) evaluating the kinetics of DNA damage induced in various organs of male mice; (4) examining the applicability of the assay to peripheral blood leukocytes obtained from humans exposed to genotoxic agents; and (5) comparing the levels of DNA damage in the organs of mice collected at an EPA Superfund site. Based on the results obtained, the technique will provide, with greater sensitivity than any other method currently available, data on the induction and persistence of organ-specific levels of DNA damage resulting from environmental exposure to genotoxic pollutants.
"December 1990." "EPA/600/4-90/034." "United States Environmental Protection Agency." Includes bibliographical references.