Record Display for the EPA National Library Catalog


Main Title Increasing ARCAT (Trade Name) Test Sensitivity for Examination of Potable Waters.
Author Isbister, Jenefir D. ; Alm, Jeanne L. ; Foutch, Roberta ; DeSouza, Alice ; Wentsel, Randall S. ;
CORP Author Atlantic Research Corp., Alexandria, VA.;Municipal Environmental Research Lab., Cincinnati, OH.
Year Published 1982
Report Number 49-5790; EPA-68-03-2914; EPA-600/2-82-025;
Stock Number PB82-196163
Additional Subjects Potable water ; Bacteriophages ; Filtration ; Tests ; Sensitivity ; Disinfection ; Ozone ; Chlorine inorganic compounds ; Water quality ; Municipalities ; Research ; Water pollution ; Water pollution detection ; Coliphages ; Zeta Plus 60S filters ; Drinking water ; ARCAT
Library Call Number Additional Info Location Last
NTIS  PB82-196163 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 101p
The primary objective of this research project was to increase the sensitivity of the ARCAT method to detect one coliphage per 100 ml of water sample. Several concentration techniques were evaluated and three methods selected for in-depth study. Two of the methods selected for in-depth study were filter concentration techniques. The third method involved amplification of coliphages by addition of nutrients and a host culture followed by incubation to allow a lytic burst cycle to occur. Of these techniques, the Zeta Plus 60S filtration technique proved to be the optimum method. Coliphage adsorption with this filter range from 80-100%. Coliphage recoveries from the filters were > or = 90%. With this concentration method, test results can be obtained within 6 1/2 hours with a sensitivity of 1 coliphage per 100 ml.