| Main Title |
Method development and preliminary applications for leptospira spirochetes in water samples / |
| Author |
M. WALKER
|
| Other Authors |
|
| CORP Author |
Nevada Univ., Reno.; Environmental Protection Agency, Research Triangle Park, NC. National Homeland Security Research Center. |
| Publisher |
U.S. Environmental Protection Agency, |
| Year Published |
2008 |
| Report Number |
EPA/600/R-08/017 |
| Stock Number |
PB2008-108874 |
| Subjects |
Leptospira spirochetes ;
Decontamination (from gases, chemicals, etc) ;
Drinking water--Analysis
|
| Additional Subjects |
Leptospira ;
Water sampling ;
Infectious diseases ;
Zoontic diseases ;
Humans ;
Animals ;
Contamination ;
Detection ;
Antibodies ;
Occurrence ;
Recrational areas ;
Watersheds ;
Hawaiian Islands ;
Leptospira spirochetes
|
| Internet Access |
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB2008-108874 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
[30] : digital, PDF file |
| Abstract |
Leptospirosis is an increasingly important zoonosis that infects humans and animals through contact with contaminated water. The primary challenges for carrying out water sampling for Leptospira spirochetes include isolation, concentration, and quantitative detection of small numbers of target organisms in water. The objectives of this work were to (1) develop a protocol to apply fluorescently labeled antibodies to water samples to detect virulent serovars of Leptospira, (2) develop a protocol to isolate Leptospira spirochetes from water samples, and (3) use these techniques to assess the occurrence of Leptospira in recreational waters in watersheds on Oahu and Kauai, in the Hawaiian Islands. |
| Notes |
"EPA/600/R-08/017 "February 2008." Title taken from title screen (viewed Nov 5, 2008). |
| Contents Notes |
Leptospirosis is an increasingly important zoonosis that infects humans and animals through contact with contaminated water. The primary challenges for carrying out water sampling for Leptospira spirochetes include isolation, concentration, and quantitative detection of small numbers of target organisms in water. The objectives of this work were to 1) develop a protocol to apply fluorescently labeled antibodies to water samples to detect virulent serovars of Leptospira, 2) develop a protocol to isolate Leptospira spirochetes from water samples, and 3) use these techniques to assess the occurrence of Leptospira in recreational waters in watersheds on Oahu and Kauai, in the Hawaiian Islands. |
