||Mutational Spectrum and Recombinogenic Effects Induced by Aminofluorene Adducts in Bacteriophage M13 (Journal Version).
Ross, J. ;
Doisy, R. ;
Tang, M. S. ;
||Health Effects Research Lab., Research Triangle Park, NC. ;Texas Univ. System Cancer Center, Smithville.
Genetic recombination ;
DNA mutational analysis ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
Double stranded replicative form (RFI) DNA of bacteriophage M13mp10 has been modified in vitro to various extents with N-hydroxy-2-aminofluorene (N-OH-AF) and then transfected into E. coli cells. HPLC analysis of the modified DNA shows that only dG-C8-AF adducts are formed. Approximately 20 adducts per FRI molecule constitute one lethal event when plaque forming ability is assayed on E. coli cells which have received no prior SOS induction. The mutagenicity of dG-C8-AF adducts was assayed by measuring loss of beta-galactosidase activity as a function of adducts per molecule. A dose-dependent increase in lac(-) mutants was observed, with a four-fold increase in mutants per survivor at 30 adducts per RFI molecule. The mutants were analyzed by DNA sequencing, and occur predominately at either G or C positions which are different from those observed in the spontaneous mutant spectrum. In the assay system, dG-C8-AF adducts induce a previously-unreported recombinogenic activity. (Copyright (c) 1988 Elsevier Science Publishers B.V. (Biomedical Division.))