Record Display for the EPA National Library Catalog


Main Title Kinetics of In vivo Sister Chromatid Exchange Induction in Mouse Bone Marrow Cells in Ethylnitrosourea and Methylnitrosourea.
Author Charles, J. L. ; Jacobson-Kram, D. ; Condie, L. ; Borzelleca, J. F. ; Carchman, R. A. ;
CORP Author Virginia Commonwealth Univ., Richmond. Dept. of Pharmacology and Toxicology.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1986
Report Number EPA-R-808861; EPA/600/J-86/158;
Stock Number PB87-118501
Additional Subjects Bone marrow ; Toxicology ; Laboratory animals ; Mice ; Kinetics ; Reprints ; Sister chromatid exchange ; Urea/ethylnitroso ; Urea/methylnitroso
Library Call Number Additional Info Location Last
NTIS  PB87-118501 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
Administration of ethylnitrosourea (ENU) (20, 25, 40, 50, and 60 mg/kg body weight) or methylnitrosourea (MNU) (25, 40, 50, 60, 75, and 80 mg/kg body weight) to male CD-1 mice 2 hours after subcutaneous implantation of 5-bromo-2'-deoxyurdine (BrdUrd) pellet (55 mg) resulted in a dose-dependent increase in sister chromatid exchanges (SCE) in bone marrow cells. Treatment with ENU (50 mg/kg body weight) at several time points prior to BrdUrd implantation resulted in a biphasic curve of SCE induction indicating at least two events that result in SCEs. Treatment with ENU at the time of BrdUrd implantation and post-BrdUrd reflected a similar mechanism apparent in the preBrdUrd curve. Treatment with MNU (50 mg/kg body weight) pre- and post-BrdUrd resulted in a linear monophasic curve of SCE induction in both the pre- and post-BrdUrd time periods. The overall MNU time-course curve resembled an inverted V function indicating the mechanism of SCE induction for ENU and MNU are different.