Record Display for the EPA National Library Catalog


Main Title Kinetics of In vivo Sister Chromatid Exchange Induction in Mouse Bone Marrow Cells by Alkylating Agents: Cyclophosphamide.
Author Charles, J. L. ; Jacobson-Kram, D. ; Borzelleca, J. F. ; Carchman, R. A. ;
CORP Author Virginia Commonwealth Univ., Richmond. Dept. of Pharmacology and Toxicology.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1983
Report Number EPA-R-808861; EPA/600/J-83/268;
Stock Number PB85-150845
Additional Subjects In vivo analysis ; Laboratory animals ; Bone marrow ; Blood cells ; Toxicology ; Dosage ; Metabolism ; Mice ; Deoxyribonucleic acids ; Chromatids ; Reprints ; Cyclophosphamide
Library Call Number Additional Info Location Last
NTIS  PB85-150845 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
Administration of cyclophosphamide (5, 10, 20 and 25 mg/kg body weight) to male CD-1 mice 2 hours after subcutaneous implantation of a 5-bromo-2'-deoxyuridine (BrdU) pellet (55 mg) resulted in a dose-dependent increase in sister chromatid exchanges (SCE) in bone marrow cells. Treatment with cyclophosphamide (15 mg/kg body weight) at the time of BrdU implantation and 2, 6.5, and 13 hours post-BrdU implantation resulted in the induction of approximately 19 SCE/cell indicating that the bone marrow SCE response was independent of the time of administration. The time-dependent induction of SCE is qualitatively similar for CP and MMC both of which are bifunctional alkylating agents metabolically activated by oxidation and reduction, respectively, and suggests that these two compound may induce SCE by a similar mechanism.