Record Display for the EPA National Library Catalog


Main Title (32)p-Adduct Assay: Principle and Applications to Carcinogen-Exposed Animal and Human DNA.
Author Gupta, R. C. ; Sharma, S. ; Earley, K. ; Mohapatra, N. ; Nesnow, S. ;
CORP Author Baylor Coll. of Medicine, Houston, TX. ;M.D. Anderson Hospital and Tumor Inst., Houston, TX.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1987
Report Number EPA/600/D-87/077;
Stock Number PB87-166948
Additional Subjects Carcinogens ; Deoxyribonucleic acids ; Malignant neoplasms ; Nucleoxides ; Mutagens ; Mutations ; 32 P labeling assay ; DNA adducts
Library Call Number Additional Info Location Last
NTIS  PB87-166948 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 18p
There is growing evidence that carcinogens initiate the malignant process via specific alterations in DNA structure, i.e., the covalent binding of carcinogens to DNA bases. Thus, carcinogen-DNA adducts represent as markers for tumor initiation. Several new techniques have been reported for detecting exceptionally small quantities of adducts without requiring test chemicals to be radioactive. These methods are based on specific antibodies, fluorescence spectra, electrophore labeling, and 32P incorporation into DNA constituents. Recent developments have indicated that the (32)P labeling method is capable of measuring a wide spectra of unusually low levels .10 to the tenth power nucleotides of DNA adducts induced by a vast majority of known aromatic/hydrophobic environmental carcinogens (Gupta, 1985). The paper reviews the underlying principle of the approach, as well as some recent applications.