||Evaluation of DNA Damage in the Oral Mucosa of Tobacco Users and Non-Users by (32)P-Adduct Assay.
Chacko, M. ;
Gupta, R. C. ;
||Baylor Univ., Houston, TX. Coll. of Medicine.;Health Effects Research Lab., Research Triangle Park, NC.
DNA adducts ;
Mouth mucosa ;
Tobacco use disorder ;
||Some EPA libraries have a fiche copy filed under the call number shown.
Tobacco and its combustion products contain several known or potential human carcinogens and studies are not beginning to emerge for detecting DNA and protein adducts in tobacco users. A highly sensitive (32)P-adduct assay, capable of measuring a wide spectra of aromatic and/or hydrophobic DNA adducts, was used to evaluate tobacco use-related adducts in human oral mucosal cells. The chromatographic behavior of the oral mucosal DNA adducts by selective PE1-cellulose TLC and their extractability in 1-butanol suggest that they have an aromatic and/or hydrophobic moiety and two of the major adducts have been tentatively assigned as aromatic amine derivatives.