Record Display for the EPA National Library Catalog


Main Title In vitro biological systems
Other Authors
Author Title of a Work
Tyson, Charles A.
Frazier, John M.
Publisher Academic Press,
Year Published 1993
OCLC Number 27696519
ISBN 0124612016 (alk. paper : hardcover) 0124012024 (alk. paper : pbk.) 0124612024 (pbk.)
Subjects Toxicology--methods ; Tissue Culture ; Organ Culture ; Cell culture--Technique ; Toxicity testing--In vitro
Library Call Number Additional Info Location Last
EHBM  RA1190.M48 v.1,pt.A CEMM/ACESD Library/Narragansett,RI 01/26/2001
Collation xxi, 568 p., {1} p. of plates : ill.
Includes bibliographical references and index.
Contents Notes
Neural and neuromuscular systems -- Hippocampal slices -- Organotypic neural cultures -- Reaggregate cultures for neurotoxicological studies -- Cytotoxicity in murine neocortical cell culture -- Cultures of adult trigeminal ganglion neurons -- Adrenomedullary chromaffin cells for toxicological studies -- PC-12 cells -- Ocular system -- Corneal epithelial and endothelial cell culture -- Isolation and culture of retinal epithelium -- Respiratory system -- Isolation and culture of type II alveolar epithelial cells -- Isolation of nonciliated bronchiolar (Clara) epithelial cells from mouse lung -- Rat tracheal epithelial cell cultures for studies in toxicology and carcinogenicity -- Cardiovascular system -- Preparation of primary cultures of postnatal rat myocardial cells for toxicological studies -- Aortic endothelial and smooth muscle cell cultures -- Vessel cylinders -- Gastrointestinal system -- Gastric mucosal cell culture for toxicological study -- Small intestinal enterocytes -- Maintenance and characterization of an organ culture system for colonic mucosa. (cont) Liver slices -- Precision-cut rat liver slices in dynamic organ culture for structure-toxicity studies -- Liver: hepatocytes -- Isolation of hepatocytes by collagenase perfusion -- Preparation of primary monolayer cultures of postnatal rat liver cells for hepatotoxic assessment of xenobiotics -- Isolation and culture of hepatocytes from different laboratory species -- Isolation of human hepatocytes by biopsy perfusion methods -- Human hepatocyte cultures -- Liver: other cell systems -- Hepatocyte and spleen cell systems -- Isolation and culture of hepatic nonparenchymal cells -- Proliferating lines of rat liver epithelial cells -- Isolation and culture of liver epithelial cells from carcinogen-treated rats -- Kidney: proximal tubule fragments -- Renal proximal tubule preparations isolated from rat or rabbit kidneys without the use of collagenase -- Suspension culture of rabbit renal proximal tubules -- Perfusion method for isolation of renal proximal tubules from mammalian species -- Isolation and maintenance of milligram quantities of rabbit renal proximal straight and convoluted tubules. (cont) Kidney cell culture -- Rabbit cortical epithelial cells -- Culture of rat kidney proximal tubule epithelial cells in defined medium -- Primary culture of rat renal medullary interstitial cells -- Purification of renal cortical cell populations by percoll density-gradient centrifugation -- Renal cell lines derived from rabbit and pig kidney -- Reproductive and developmental systems -- Whole embryo culture: organogenesis of rodent embryos in vitro -- Preparation and use of sertoli-germ cell cocultures from 28-day-old rats -- Immune system -- Isolation and in vitro culture of mononuclear phagocytes -- T-lymphocyte assays -- B lymphocytes: comparison of murine and human B-cell immunocompetence -- Bone marrow stromal cell cultures -- Skin -- Penetration studies with excised human and animal skin -- Porcine skin flaps -- Keratinocytes.