Record Display for the EPA National Library Catalog


Main Title In vitro toxicity indicators
Other Authors
Author Title of a Work
Tyson, Charles A.
Frazier, John M.
Publisher Academic Press,
Year Published 1994
OCLC Number 30501005
ISBN 0124612032 (hardcover : alk. paper) 0124612040 (papercover : alk. paper)
Subjects Cells--drug effects ; Cell Death ; Biological Markers--analysis ; Indicators (Biology) ; Toxicity testing--In vitro
Library Call Number Additional Info Location Last
EHBM  RA1190.M48 v.1,pt.B CEMM/ACESD Library/Narragansett,RI 01/26/2001
Collation xx, 497 p. : ill.
Includes bibliographical references and index.
Contents Notes
Perspectives -- Cellular and molecular pathobiology of reversible and irreversible injury -- Molecular mechanisms of toxic cell death: an overview -- Membrane lipid peroxidation: cellular mechanisms and toxicological implications -- Membrane integrity and function -- Enzyme leakage as an indicator of cytoxicity in cultured cells -- Cellular K+ -- Morphological and biochemical investigations on plasma membrane blebbing during cell injury -- Morphological evaluation of gap junctional intercellular communication. Fluorescence microscopic examination of cytoskeletal elements -- Energy coupled Na+ cotransport -- Renal organic ion transport -- Endoplasmic reticulum calcium pump -- Energy metabolism -- Measurement of oxygen consumption -- ATP and other energetic parameters as indicators of cell injury -- Evaluation of glucose metabolism in vitro: altered competence of intermediary metabolism as an index of hepatotoxicity -- DNA-related indices -- Quantitation of DNA by fluorometry -- Techniques for measuring cell proliferation. Nuclease P1-enhanced 32P-postlabeling assay for aromatic carcinogen-DNA adducts -- Genomic hypomethylation -- DNA degradation: double-strand breaks -- Genotypic mutation analysis by RFLP/PCR -- Functional indices -- Gluconeogenesis in liver cells -- Measurement of protein synthesis -- Measuring collagen synthesis -- Measurement of macrophage phagocytosis using a sheep red blood cell assay -- Biochemical indices -- Cell function studies using fluorescent Ca2+ indicators -- Ca2+-bioluminescent indicators. Measurement of receptor-activated accumulation of inositol phosphates as an index of phospholipase C activity -- Covalent binding to proteins -- Glutathione, oxidized glutathione and mixed disulfides in biological samples -- Lipid peroxidation -- Thiobarbituric acid assay reactants -- Volatile hydrocarbons -- Electronically excited state formation -- Spectrophotometric detection of lipid-conjugated dienes -- Quantitation of conjugated dienes by a dieles-alder reaction -- Quantitation of conjugated dienes by second-derivative UV spectroscopy. Specialized techniques -- Centrifuge filtration separation of cells and the measurement of constituents -- Flow cytometry -- Single-cell microscopic techniques for studying toxic injury -- Measurement of macrophage and neutrophil chemotaxis -- Two-dimensional electrophoresis as applied to problems of in vitro and in vivo toxicology.