Record Display for the EPA National Library Catalog


Main Title DNA Strand Breaks Induced in Cultured Human and Rodent Cells by Chlorohydroxyfuranones, Mutagens Isolated from Drinking Water.
Author Chang, L. W. ; Daniel, F. B. ; DeAngelo, A. B. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-92/120;
Stock Number PB92-164904
Additional Subjects Toxicology ; DNA damage ; Mutagens ; Potable water ; Cultured cells ; Disinfection ; Rats ; Humans ; Liver ; Glutathione ; Salmonella typhimurium ; Cell survival ; Lactate dehydrogenase ; Metabolic activation ; Reprints ; Chlorohydroxyfuranones
Library Call Number Additional Info Location Last
NTIS  PB92-164904 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 14p
Chlorohydroxyfuranones, by-products of chlorine disinfection and drinking water contaminants, are shown to produce DNA strand breaks in human and rodent cells. One chlorohydroxyfuranone, 3-chloro-4-dichloromethyl-5-hydroxy-2(5H)-furanone(MX), a potent bacterial mutagen, induces 232 + or - 89 DNA strand breaks/(cell-micromole) in human CCRF-CEM cells over a concentration range of 4.4 to 220 micromole. This constitutes a DNA damage potency comparable to dimethylsulfate (DMS). By comparison, 3,4-dichloro-5-hydroxy-2(5H)-furanone (MA), another chlorohydroxyfuranone which is approximately four orders of magnitude less mutagenic than MX in Salmonella typhimurium strain TA 100, is only about tenfold less potent as an inducer of DNA strand breaks in these cells, i.e., 18.2 + or - 3.1 strand breaks/(cell-micromole). The DNA strand-breaking potential of MX is inactivated by prior incubation with a rat liver S9 homogenate. In addition, both chlorohydroxyfuranones are ineffective at producing DNA strand breaks in primary rate hepatocytes (PRH) at concentrations below those which produce cytotoxicity as assessed by release of the cellular enzyme lactate dehydrogenase (LDH).