Record Display for the EPA National Library Catalog


Main Title DNA Binding and Adduct Formation of Aflatoxin B1 in Cultured Human and Animal Tracheobronchial and Bladder Tissues.
Author Stoner, G. D. ; Daniel, F. B. ; Schenck, K. M. ; Schut, H. A. J. ; Sandwisch, D. W. ;
CORP Author Medical Coll. of Ohio at Toledo.;Health Effects Research Lab., Cincinnati, OH.
Year Published 1982
Report Number EPA-R-807670; EPA/600/J-82/437;
Stock Number PB84-243989
Additional Subjects Deoxyribonucleic acids ; Chemical analysis ; Bladder ; Respiratory system ; Carcinogens ; Tissue culture ; Laboratory animals ; Humans ; Exposure ; Chromatographic analysis ; Chemical bonds ; Separation ; Environmental surveys ; Reprints ; Aflatoxins ; High pressure liquid chromatography
Library Call Number Additional Info Location Last
NTIS  PB84-243989 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
DNA binding and adduct formation of aflatoxin B1 (AFB1) was studied in cultured bladder and tracheobronchial explants from human, monkey, dog, hamster and rat. Explants were exposed to (3H)AFB1 (1 micrometer final concentration) in PFHR-4 medium (pH 7.4) without serum for 24 h, after which epithelial cell DNA was isolated by hydroxylapatite chromatography. These binding levels were not correlated with the relative susceptibilities of these species to AFB1 hepatocarcinogenesis, in that the hamster and the dog are insensitive, but exhibited the highest binding, while the susceptible species, the rat and the monkey, had lower binding. After acid hydrolysis of the isolated DNA, the (3H)AFB1-DNA adducts were separated by high-pressure liquid chromatography. In some cases small amounts (0-8%) of unknown, polar adducts could be detected.