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Main Title Micronuclei in Binucleated Lymphocytes of Mice Following Exposure to Gamma Radiation.
Author Erexson, G. L. ; Kligerman, A. D. ; Halperin, E. C. ; Honore, G. M. ; Allen., J. W. ;
CORP Author Environmental Protection Agency, Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Duke Univ. Medical Center, Durham, NC.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-89/232;
Stock Number PB90-145988
Additional Subjects Gamma radiation ; Lymphocytes ; Mice ; Exposure ; Dosimetry ; In vitro analysis ; In vivo analysis ; Deoxyribonucleic acids ; Mutations ; Reprints ; Mononuclear leukocytes ; Micronucleus tests ; Mutagenicity tests ; Dose-response relationships ; Cobalt radioisotopes
Library Call Number Additional Info Location Last
NTIS  PB90-145988 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
Experiments were designed to investigate the induction of micronuclei (MN) in mouse peripheral blood lymphocytes (PBLs) after in vitro or in vivo exposure to (60)Co gamma radiation. For the in vitro experiments, 4 ml of blood from male C57BL/6J mice were either irradiated in 6 ml Falcon culture tubes as whole blood or isolated to obtain mononuclear leukocytes (MNLs) that were pelleted by centrifugation and then irradiated in RPMI 1640. For the in vivo analysis mice received whole body irradiation, blood was obtained by cardiac puncture, and the MNLs were isolated for each mouse. Exposures were at a rate of 0.82 to 0.90 Gy/min to yield doses of 0.5, 1, 2, 3, or 4 Gy. MNLs were cultured using cytochalasin B for MN analysis in binucleated PBLs. There was a significant dose-dependent increase in MN observed at all doses. Dose-response curves for the in vivo and in vitro whole blood experiment were not significantly different. However, for isolated pelleted MNLs irradiated in vitro, the MN frequency at 4 Gy was less than half that seen in the in vivo experiment. The large difference in MN response is thought to be due to the radioprotective effect of hypoxia. (Copyright (c) 1989 Alan R. Liss, Inc.)