Grantee Research Project Results
2013 Progress Report: Predicting Food Protein Allergenicity Using a Mouse Model
EPA Grant Number: R834822Title: Predicting Food Protein Allergenicity Using a Mouse Model
Investigators: Gangur, Venugopal
Institution: Michigan State University
EPA Project Officer: Aja, Hayley
Project Period: September 15, 2010 through September 14, 2014
Project Period Covered by this Report: September 15, 2012 through September 14,2013
Project Amount: $424,919
RFA: Approaches to Assessing Potential Food Allergy from Genetically Engineered Plants (2009) RFA Text | Recipients Lists
Research Category: Human Health
Objective:
1) Determine the NOAEL and the LOAEL of food proteins with high vs. low/no allergenic potential in a mouse model.
2) Determine the immune, clinical and physiological responses to isolated pure food proteins with high vs. low/no allergenic potential in this model.
Progress Summary:
Future Activities:
We plan to evaluate the allergenicity of purified protein ovalbumin, establish oral-dose response curves and percent responder curves, and estimate oral elicitation threshold doses (LOAEL and NOAEL) in TS/OE mouse model. Based on the mouse model data and using published methods, we will estimate the human equivalent threshold doses for ovalbumin. Subject to availability of adequate funds, we plan to evaluate allergenicity of another purified protein, rubisco, in this mouse model. We plan to submit a manuscript for publication and present an abstract for presentation during the American Association of Immunologists Annual Meeting to be held in May 2014.
Journal Articles:
No journal articles submitted with this report: View all 19 publications for this projectSupplemental Keywords:
food allergy, food safety, allergen, systemic anaphylaxis, exposure, hazard assessment, mouse model, threshold elicitation dose, NOAEL, LOAEL, potency testing, dose-response, genetic engineering, allergenicityRelevant Websites:
http://www.hesiglobal.org/files/19-Gangur-Mouse%20Model-Hazelnut-May%202013.pdf Exit
Progress and Final Reports:
Original AbstractThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.