Grantee Research Project Results
2012 Progress Report: Predicting Food Protein Allergenicity Using a Mouse Model
EPA Grant Number: R834822Title: Predicting Food Protein Allergenicity Using a Mouse Model
Investigators: Gangur, Venugopal
Institution: Michigan State University
EPA Project Officer: Aja, Hayley
Project Period: September 15, 2010 through September 14, 2014
Project Period Covered by this Report: September 15, 2011 through September 14,2012
Project Amount: $424,919
RFA: Approaches to Assessing Potential Food Allergy from Genetically Engineered Plants (2009) RFA Text | Recipients Lists
Research Category: Human Health
Objective:
The specific objectives for this project are:
(1) Determine the NOAEL and the LOAEL of plant-derived food proteins with high vs. low/no allergenic potential in a mouse model.
(2) Determine the immune, clinical and physiological responses to isolated pure food proteins with high vs. low/no allergenic potential in this model.
Progress Summary:
(1) Determination of oral elicitation threshold doses (NOAEL and LOAEL) for hazelnut protein in transdermal sensitization/oral elicitation (TS/OE) mouse model; (2) Estimation of human equivalent doses of hazelnut based on mouse threshold doses and published methodology; (3) To produce in-house pigeon pea protein extract, evaluate allergenic potential, establish oral elicitation dose-response and percent responder curve for pigeon-pea protein extract in this model; and (4) To optimize a method for preparing high-quality sesame seed protein extract for using during the next year of the project.
Future Activities:
We plan to further work on pigeon pea protein and sesame seed protein during the next year; we expect to produce high-quality sesame seed protein for use in the project and evaluate its allergenic potential in this model; we plan to establish the oral elicitation dose responses, establish the percent responder curve and determine the threshold oral elicitation doses (LOAEL and NOAEL) for sesame seed protein; using the mouse data, we will estimate human equivalent threshold doses for sesame seed protein; submit a manuscript for publication and present an abstract during the AAI 2013 annual meeting.
Journal Articles:
No journal articles submitted with this report: View all 19 publications for this projectSupplemental Keywords:
food allergy, food safety, allergen, systemic anaphylaxis, exposure, hazard assessment, mouse model, threshold elicitation dose, NOAEL, LOAEL, potency testing, dose-response, genetic engineering, allergenicityProgress and Final Reports:
Original AbstractThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.