Grantee Research Project Results
Final Report: Genomic Database for Cryptosporidium spp.
EPA Grant Number: R825148Title: Genomic Database for Cryptosporidium spp.
Investigators: Upton, Steve J. , Dykstra, Christine C. , Blagburn, Byron L.
Institution: Kansas State University , Auburn University Main Campus
EPA Project Officer: Packard, Benjamin H
Project Period: September 23, 1996 through September 22, 1999 (Extended to September 22, 2000)
Project Amount: $304,755
RFA: Drinking Water (1996) RFA Text | Recipients Lists
Research Category: Water
Objective:
Using molecular biology techniques, the key feature of this grant was to develop genomic libraries for a variety of isolates of Cryptosporidium parvum, and a variety of additional species within the genus. These libraries would be made accessible to investigators who wish to use the libraries to develop genetic probes for specific regions within the parasite genome. The advantage of these libraries was stated as threefold. First, the investigator could sequence and study a specific area of the genome of their choosing, and from a wide variety of species and isolates. Second, the investigator would no longer need to rely on the error prone sequences from various species and isolates, such as the 18s ribosomal subunit sequences, submitted by multiple laboratories into the national databases. Third, the investigator could develop specific, rather than random, primers for developing diagnostic tests. Collectively, the completion of this project should allow for a more rapid development of molecular based diagnostic tools for Cryptosporidium spp. so that water quality standards can more easily be met.
Summary/Accomplishments (Outputs/Outcomes):
The proposed project did not represent a research project per se but primarily represented construction and deposition of type (DNA) material into an accredited repository. Twenty genomic libraries were originally proposed; 10 from Kansas State University and 10 from Auburn University. A total of 20 were submitted to the American Type Culture Collection. In addition to the libraries being deposited in the American Type Culture Collection, one important taxonomic revision resulted. Careful analysis of the 18s ribosomal DNA subunits, hsp70 genes, and internal transcribed spacer 1 (ITS1) regions of the various species revealed the large, gastric Cryptosporidium "muris"-like isolates really to consist of two distinct species. Thus, a new species, Cryptosporidium andersoni, was created to accommodate the large, abomasal Cryptosporidium sp. of cattle. The name Cryptosporidium. muris was retained for the large murine species plus a few additional isolates. Results of these taxonomic studies were published by Morgan, et al., who provided the molecular basis for the taxonomic revision, and Lindsay et al., who provided further morphologic and developmental biology justification.
Journal Articles on this Report : 9 Displayed | Download in RIS Format
| Other project views: | All 13 publications | 9 publications in selected types | All 9 journal articles |
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Chung PA, Johnson J, Khramtsov NV, Upton SJ. Cloning and molecular characterization of a gene encoding a Cryptosporidium parvum putative 20s proteasome β1-type subunit. Mitochondrial DNA 2000;11(3-4):309-314. |
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Fayer R, Morgan U, Upton SJ. Epidemiology of Cryptosporidium: transmission, detection and identification. International Journal for Parasitology 2000;30(12-13):1305-1322. |
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Khramtsov NV, Upton SJ. High-temperature inducible cell-free transcription and replication of double-stranded RNAs within the parasitic protozoan Cryptosporidium parvum. Virology 1998;245(2):331-337. |
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Khramtsov NV, Chung PA, Dykstra CC, Griffiths JK, Morgan UM, Arrowood MJ, Upton SJ. Presence of double-stranded RNAs in human and calf isolates of Cryptosporidium parvum. Journal of Parasitology 2000;86(2):275-282. |
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Khramtsov NV, Upton SJ. Association of RNA polymerase complexes of the parasitic protozoan Cryptosporidium parvum with virus-like particles: heterogeneous system. Journal of Virology 2000;74(13):5788-5795. |
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Lindsay DS, Upton SJ, Owens DS, Morgan UM, Mead JR, Blagburn BL. Cryptosporidium andersoni n. sp. (Apicomplexa: Cryptosporiidae) from cattle, Bos taurus. Journal of Eukaryotic Microbiology 2000;47(1):91-95. |
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Lindsay DS, Woods KM, Upton SJ, Blagburn BL. Activity of decoquinate against Cryptosporidium parvum in cell cultures and neonatal mice. Veterinary Parasitology 2000;89(4):307-311. |
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Morgan UM, Xiao L, Monis P, Sulaiman I, Pavlasek I, Blagburn B, Olsen M, Upton SJ, Khramtsov NV, Lal A, Elliot A, Thompson RCA. Molecular and phylogenetic analysis of Cryptosporidium muris from various hosts. Parasitology 2000;120(5):457-464. |
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Woods KM, Tilley M, Iselp A, Upton SJ, Montelone BA, Khramtsov NV. Sequence of the gene encoding hsp90e from Cryptosporidium parvum. Mitochondrial DNA 1999;10(4-5):339-342. |
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Supplemental Keywords:
Cryptosporidium, coccidia, drinking water, groundwater, human health, animal, mammalian, organism, genetic polymorphisms, pathogens, public good, biology, zoology, epidemiology, pathology, genetics, surveys, analytical, agriculture., RFA, Scientific Discipline, Water, Environmental Chemistry, Genetics, Health Risk Assessment, Analytical Chemistry, Drinking Water, pathogens, public water systems, Safe Drinking Water, monitoring, microbial risk assessment, detection, human health effects, exposure and effects, exposure, analytical methods, cryptosporidium , genomic database, public health, treatment, microbial risk management, emerging pathogens, DNA libraries, drinking water contaminants, infectivityRelevant Websites:
Multiple Web sites have been created either all, or in part, as a component of this grant. These sites, which reside at Kansas State University, include the following:
Cryptosporidium and Cryptosporidiosis Exit
Commercial reagents for Cryptosporidium research Exit
Cryptosporidium oocyst measurements Exit
Taxonomic chronology of Cryptosporidium Exit
Waterborne/foodborne outbreaks of Exit
Progress and Final Reports:
Original AbstractThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.