Grantee Research Project Results
Final Report: Innate Immune Response of an Aquatic Vertebrate Model to Manufactured Nanoparticles Assessed Using Genomic Markers
EPA Grant Number: R833319Title: Innate Immune Response of an Aquatic Vertebrate Model to Manufactured Nanoparticles Assessed Using Genomic Markers
Investigators: Klaper, Rebecca , Goetz, Frederick , Chen, Jian
Institution: University of Wisconsin - Madison
EPA Project Officer: Hahn, Intaek
Project Period: April 1, 2008 through April 15, 2011
Project Amount: $398,810
RFA: Exploratory Research: Nanotechnology Research Grants Investigating Environmental and Human Health Effects of Manufactured Nanomaterials: a Joint Research Solicitation-EPA, NSF, NIOSH, NIEHS (2006) RFA Text | Recipients Lists
Research Category: Nanotechnology , Safer Chemicals
Objective:
The overall objective of this project is to assess the innate immune reaction of an aquatic model, the rainbow trout, to manufactured nanomaterials of varying chemistries at levels not inducing cellular toxicity. This research will create a mechanism with which to test other nanomaterials, provide data to support ecological risk assessments, and ultimately inform decisions as to which materials will be the safest to industrialize and use with respect to aquatic environments.
Summary/Accomplishments (Outputs/Outcomes):
Conclusions:
We have found that, despite a lack of change in cell viability, nanomaterials vary in their stimulation of the immune response as indicated by QPCR of genes indicative of an inflammatory response such as IL1. All nanomaterials we have tested cause an increase in candidate proinflammatory genes that is equivalent to stimulation of positive controls at the highest concentration of 10 ug/mL, but as the concentration drops to 1or .1 ug/mL, there are differences in inflammatory responses with nanomaterials with differing chemistries and in a dose dependent fashion. There are a couple of nanomaterials that appear to be immune suppressive at all exposures tested. This would indicate that the dose that ultimately enters the organism will be extremely important to determine potential immune responses.
A microarray specifically designed to probe the global immune response of macrophages was used to examine differences in gene expression across nanomaterial treatments and to compare these expression patterns to traditional immune stimulants from viruses and bacteria. Even though each nanomaterial caused a similar inflammatory response in macrophage cells at 5 ug/mL exposures, the global gene expression differed with functionalization of nanomaterial and with core structure. This was mainly in the form of the types of genes expressed in each nanomaterial treatment indicating an overall difference in response to each nanomaterial.
Journal Articles on this Report : 1 Displayed | Download in RIS Format
Other project views: | All 18 publications | 1 publications in selected types | All 1 journal articles |
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Type | Citation | ||
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Klaper R, Arndt D, Setyowati K, Chen J, Goetz F. Functionalization impacts the effects of carbon nanotubes on the immune system of rainbow trout, Oncorhynchus mykiss. Aquatic Toxicology 2010;100(2):211-217. |
R833319 (2009) R833319 (Final) |
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Supplemental Keywords:
Risk assessment, dose response, exposure, immunology, ecological effects, nanotoxicology, immunotoxicology, genomics, biomarkers, Health, Scientific Discipline, Environmental Chemistry, Health Risk Assessment, Risk Assessments, biological pathways, nanochemistry, aquatic ecosystem, bioavailability, nanotechnology, environmental risks, manufactured nanomaterials, nanomaterials, toxicologic assessment, biogeochemistry, nanoparticle toxicity, cellular response to nanoparticles, bioaccumulationRelevant Websites:
University of Wisconson - Milwaukee School of Freshwater Sciences: Rebecca D. Klaper Exit
Progress and Final Reports:
Original AbstractThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.