Grantee Research Project Results
2009 Progress Report: Innate Immune Response of an Aquatic Vertebrate Model to Manufactured Nanoparticles Assessed Using Genomic Markers
EPA Grant Number: R833319Title: Innate Immune Response of an Aquatic Vertebrate Model to Manufactured Nanoparticles Assessed Using Genomic Markers
Investigators: Klaper, Rebecca , Goetz, Frederick , Chen, Jian
Institution: University of Wisconsin - Madison
EPA Project Officer: Hahn, Intaek
Project Period: April 1, 2008 through April 15, 2011
Project Period Covered by this Report: April 1, 2009 through March 31,2010
Project Amount: $398,810
RFA: Exploratory Research: Nanotechnology Research Grants Investigating Environmental and Human Health Effects of Manufactured Nanomaterials: a Joint Research Solicitation-EPA, NSF, NIOSH, NIEHS (2006) RFA Text | Recipients Lists
Research Category: Nanotechnology , Safer Chemicals
Objective:
The overall objective of this project is to assess the innate immune reaction of an aquatic model, the rainbow trout, to manufactured nanomaterials of varying chemistries at levels not inducing cellular toxicity. This research will create a mechanism with which to test other nanomaterials, provide data to support ecological risk assessments, and ultimately inform decisions as to which materials will be the safest to industrialize and use with respect to aquatic environments.
Progress Summary:
- Trout macrophages are proving to be a sensitive tool to investigate the effects of nanoparticles (NP) on gene expression.
- Side-chains used on NPs may have significant effects on their own. For example, cyclodextrin is a stimulator of inflammatory gene expression in trout macrophages.
- Surfactants used to solubilize NPs may have significant effects on gene expression. Deoxycholate and tetrahydrofuran are both stimulatory of inflammatory gene expression in trout macrophages.
- C60 fullerenes stimulate inflammatory gene expression in trout macrophages and functionalization of these nanomaterials may decrease this effect.
- There is a dose-response effect of nanomaterials on the immune response of trout macrophages with differences seen in the inflammatory responses at levels at or below 1 µg/ml.
Journal Articles on this Report : 1 Displayed | Download in RIS Format
Other project views: | All 18 publications | 1 publications in selected types | All 1 journal articles |
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Type | Citation | ||
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Klaper R, Arndt D, Setyowati K, Chen J, Goetz F. Functionalization impacts the effects of carbon nanotubes on the immune system of rainbow trout, Oncorhynchus mykiss. Aquatic Toxicology 2010;100(2):211-217. |
R833319 (2009) R833319 (Final) |
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Supplemental Keywords:
risk assessment, dose response, exposure, immunology, ecological effects, nanotoxicology, immunotoxicology, genomics, biomarkers, Health, Scientific Discipline, Environmental Chemistry, Health Risk Assessment, Risk Assessments, biological pathways, nanochemistry, aquatic ecosystem, bioavailability, nanotechnology, environmental risks, manufactured nanomaterials, nanomaterials, toxicologic assessment, biogeochemistry, nanoparticle toxicity, cellular response to nanoparticles, bioaccumulationRelevant Websites:
Progress and Final Reports:
Original AbstractThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.