Grantee Research Project Results
2009 Progress Report: Safety Assessment of Dietary Proteins for Allergenicity Using an Adjuvant-Free Mouse Model
EPA Grant Number: R833133Title: Safety Assessment of Dietary Proteins for Allergenicity Using an Adjuvant-Free Mouse Model
Investigators: Gangur, Venugopal , Tempelman, Robert J.
Institution: Michigan State University
EPA Project Officer: Hahn, Intaek
Project Period: October 1, 2006 through September 30, 2009 (Extended to March 31, 2010)
Project Period Covered by this Report: October 1, 2008 through September 30,2009
Project Amount: $447,774
RFA: Biotechnology: Potential Allergenicity of Genetically Engineered Foods (2006) RFA Text | Recipients Lists
Research Category: Human Health
Objective:
Food allergy is a serious public health problem of international significance that is growing world-wide. Specific reasons for this increase in food allergy are not completely understood at present. Whether genetically engineered (GE) foods contribute to this problem is largely unknown. Assessment of allergenic potential of GE foods is a major challenge facing the regulatory agencies and the agro-biotech industry. Although expert panels suggest the use of animal models as one of the methods for assessment of allergenic potential of GE foods, validated animal models are not available at present. The goal of this project is to test the validity of a novel adjuvant-free mouse model of food allergy that we have previously reported (Birmingham et al., 2007; Navuluri et al., 2006), for this purpose. Here we have been testing the hypothesis that this mouse model will be highly reliable in discerning food proteins that have or do not have intrinsic allergenic sensitization potential in humans. The two specific objectives for this project are to: (1) determine the positive predictive value (i.e., sensitivity) of the mouse-model; and (2) determine the negative predictive value (i.e., specificity) of the mouse model for testing allergenicity of dietary proteins.
Progress Summary:
The data obtained so far from this project demonstrate that: (1) the allergenicity readouts in this mouse model are dependent on the number of exposures to the allergenic protein; (2) seven human allergenic proteins (hazelnut, cashew nut, sesame seed, milk, shellfish, egg, fish) produce all three readouts of allergenicity in this mouse model; (3) five human non-allergenic proteins (kidney bean, Pinto bean, blue berry, sorghum, pigeon pea) were non-allergenic in this model based on clinical scores and hypothermia readouts; and (4) Amaranth seed protein that has no history of human allergenicity and therefore presumed to be non-allergenic, tested positive for all three readouts of allergenicity. These data suggest that this model has a very promising positive and negative predictive value for the evaluation of dietary proteins for allergenicity.
A positive outcome from this project will be a validated adjuvant-free mouse model for allergenicity hazard identification of novel dietary proteins, including pesticidal proteins used in genetically engineered foods. This outcome will enhance our ability to quantitatively assess allergenic potency of novel proteins relative to known allergenic and non-allergenic proteins in a rational, reproducible, and cost effective manner. Finally, this research will advance our basic knowledge on how environmental dietary proteins might interact with the immune system and adversely impact human health.
Future Activities:
We plan to do further data analysis and prepare manuscripts/abstracts for publication.References:
Five papers published or accepted for publication; two in preparation; four abstracts published/presented; one abstract planned; three oral presentations during conference/workshop/University.
Papers: 1) Parvataneni et al 2009 Int Arch Allergy Immunol 2009;149:299-304; 2) Parvataneni et al Int J Immunogenet. 2009 Oct;36(5):261-7; 3) Gonipeta et al 2009 J. Dairy Sci. 2009. 92:4738-4744; 4) Gonipeta et al Long-term characteristics of hazelnut allergy in an adjuvant-free mouse model. Int Arch Allergy Immunol, In Press; 5) Kelly and Gangur. Sex-disparity in food allergy: evidence from the PubMed database. J Allergy. In Press; 6) Parvataneni et al Mechanism of walnut induced shock in mice (In Preparation); 7) Gonipeta et al Long-term characteristics of milk allergic responses in an adjuvant free mouse model (In preparation)
Abstracts and presentations:
· Three abstracts presented as posters during the American Association of Immunologists, Annual meetings held during 2007, 2008, 2009
· Oral presentation of data during the workshop: ILSI, HESI Food Allergenicity Workshop held in
· Oral presentation of data during the symposium on food allergy, Society of Toxicology Annual Meeting, March, 2009, · Oral presentation of data, HNF 892 Seminar Series,
· Abstracts planned: American Association of Immunologists, Annual meeting to be held May 2010 in
Journal Articles on this Report : 4 Displayed | Download in RIS Format
Other project views: | All 23 publications | 8 publications in selected types | All 8 journal articles |
---|
Type | Citation | ||
---|---|---|---|
|
Gonipeta B, Parvataneni S, Tempelman RJ, Gangur V. An adjuvant-free mouse model to evaluate the allergenicity of milk whey protein. Journal of Dairy Science 2009;92(10):4738-4744. |
R833133 (2009) R833133 (Final) |
Exit Exit Exit |
|
Gonipeta B, Parvataneni S, Paruchuri P, Gangur V. Long-term characteristics of hazelnut allergy in an adjuvant-free mouse model. International Archives of Allergy and Immunology 2010;152(3):219-225. |
R833133 (2009) R833133 (Final) |
Exit |
|
Kelly C, Gangur V. Sex-disparity in food allergy: evidence from the PubMed database. Journal of Allergy 2009;2009:159845. |
R833133 (2009) R833133 (Final) |
Exit Exit |
|
Parvataneni S, Birmingham NP, Gonipeta B, Gangur V. Dominant, non-MHC genetic control of food allergy in an adjuvant-free mouse model. International Journal of Immunogenetics 2009;36(5):261-267. |
R833133 (2009) R833133 (Final) |
Exit |
Supplemental Keywords:
food allergy, food safety, allergen, systemic anaphylaxis, exposure, hazard assessment, mouse model, potency testing, dose-response, genetic engineering, allergenicity;, Health, Scientific Discipline, Health Risk Assessment, Risk Assessments, Allergens/Asthma, Biochemistry, dietary protein, food allergenicity, genetically engineered food, oral allergy syndrome, bioinformatics, allergic responseProgress and Final Reports:
Original AbstractThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.