Grantee Research Project Results
2007 Progress Report: Conducting-Polymer Nanowire Immunosensor Arrays for Microbial Pathogens
EPA Grant Number: GR832375Title: Conducting-Polymer Nanowire Immunosensor Arrays for Microbial Pathogens
Investigators: Mulchandani, Ashok , Yates, Marylynn V. , Myung, Nosang V. , Chen, Wilfred
Current Investigators: Mulchandani, Ashok , Chen, Wilfred , Yates, Marylynn V. , Myung, Nosang V.
Institution: University of California - Riverside
EPA Project Officer: Hahn, Intaek
Project Period: June 1, 2005 through March 31, 2008 (Extended to March 31, 2009)
Project Period Covered by this Report: June 1, 2008 through May 30,2009
Project Amount: $320,000
RFA: Greater Research Opportunities: Research in Nanoscale Science Engineering and Technology (2004) RFA Text | Recipients Lists
Research Category: Nanotechnology , Safer Chemicals
Objective:
A promising approach for the direct (label-free) electrical detection of biological macromolecules uses one-dimensional (1-D) nanostructures such as nanowires and nanotubes, configured as field-effect transistors that change conductance upon binding of charged macromolecules to receptors linked to the device surfaces. Combined with simple, rapid and label-free detection, potentially to single molecule, these nanosensors are also attractive due to the small size, low power requirement and most of all possibility of developing high density arrays for simultaneous analyses of multiple species. Although current nanosensor based on carbon nanotubes and silicon nanowires has elucidated the power of 1-D nanostructures as biosensors, they have low throughput and limited controllability and are unattractive for fabrication of high-density sensor arrays. More importantly, surface modifications, typically required to incorporate specific antibodies, have to be performed post-synthesis and post-assembly, limiting our ability to individually address each nanostructured sensing elements with the desired specificity.
The overall objective of the proposed research is to develop a novel technique for the facile fabrication of bioreceptor (antibody) -functionalized nanowires that are individually addressable and scalable to high-density biosensor arrays and to demonstrate its application for label-free, real-time, rapid, sensitive and cost-effective detection of multiple pathogens in water. Electropolymerization of conducting polymers between two contact electrodes is a versatile method for fabricating nanowire biosensor arrays with the required controllability. The benign conditions of electropolymerization enable the sequential deposition of conducting-polymer nanowires with embedded antibodies onto a patterned electrode platform, providing a revolutionary route to create a “truly” high-density and individually addressable nanowire biosensor arrays. The nanowire immunosensor arrays utility will be used to simultaneously quantify three important model pathogens, poliovirus, hepatitis A virus and rotarvirus.
Progress Summary:
The goal of this year was to investigate different methods for biological functionalization of nanowires. Earlier we had demonstrated incorporation of biomolecule by entrapment during electrochemical polymerization. As an alternative, during the past year we have developed a new simple and cost-effective, all-electrochemical method to fabricate and assemble single conducting polymer nanowire based biosensors. Polypyrrole (Ppy) nanowires were synthesized by electrochemical polymerization using alumina template. Single nanowire chemoresistive sensor device was assembled using AC dielectrophoretic alignment followed by maskless anchoring on a pair of gold electrodes separated by 3 mm. The nanowires were functionalized with antibodies against cancer biomarker CA-125 and applied for sensitive and selective detection of CA-125.
Future Activities:
In the coming year research will be performed on demonstrating the applications of the recently developed biosensor fabrication method to demonstrate detection of DNA and bacteriophages.
Journal Articles on this Report : 5 Displayed | Download in RIS Format
| Other project views: | All 9 publications | 9 publications in selected types | All 7 journal articles |
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Aravinda CL, Cosnier S, Chen W, Myung NV, Mulchandani A. Label-free detection of cupric ions and histidine-tagged proteins using single poly(pyrrole)-NTA chelator conducting polymer nanotube chemiresistive sensor. Biosensors and Bioelectronics 2009;24(5):1451-1455. |
GR832375 (2007) |
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Bangar MA, Shirale DJ, Chen W, Myung NV, Mulchandani A. Single conducting polymer nanowire chemiresistive label-free immunosensor for cancer biomarker. Analytical Chemistry 2009;81(6):2168-2175. |
GR832375 (2007) |
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Ramanathan K, Bangar MA, Yun M, Chen W, Mulchandani A, Myung NV. In situ fabrication of single poly(methyl pyrrole) nanowire. Electroanalysis 2007;19(7-8):793-797. |
GR832375 (2006) GR832375 (2007) |
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Wanekaya AK, Lei Y, Bekyarova E, Chen W, Haddon R, Mulchandani A, Myung NV. Fabrication and properties of conducting polypyrrole/SWNT-PABS composite films and nanotubes. Electroanalysis 2006;18(11):1047-1054. |
GR832375 (2005) GR832375 (2007) |
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Wanekaya AK, Chen W, Myung NV, Mulchandani A. Nanowire-based electrochemical biosensors. Electroanalysis 2006;18(6):533-550. |
GR832375 (2005) GR832375 (2007) |
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Supplemental Keywords:
Nanosensors; viruses; bacteria; monitoring; water;, Sustainable Industry/Business, RFA, Ecosystem Protection/Environmental Exposure & Risk, Scientific Discipline, Water, POLLUTANTS/TOXICS, Chemical Engineering, Environmental Chemistry, Engineering, Chemistry, & Physics, Monitoring/Modeling, New/Innovative technologies, Environmental Engineering, Environmental Monitoring, Water Pollutants, electrochemical polymerization, homeland security, environmental measurement, carbon nanotubes, nanowires, nanoengineering, nanotechnology, nanosensors, analytical chemistry, continous monitoring, microbial pathogens, continuous monitoring, nanocontact sensor, immunosensor arrays, bioterrorismProgress and Final Reports:
Original AbstractThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.