Grantee Research Project Results
Kinetics of Clearance and Relocation of Insoluble Ultrafine Iridium Particles From the Rat Lung Epithelium to Extrapulmonary Organs and Tissues (Pilot Project)
EPA Grant Number: R827354C007Subproject: this is subproject number 007 , established and managed by the Center Director under grant R827354
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: Health Effects Institute (2000 — 2005)
Center Director: Greenbaum, Daniel S.
Title: Kinetics of Clearance and Relocation of Insoluble Ultrafine Iridium Particles From the Rat Lung Epithelium to Extrapulmonary Organs and Tissues (Pilot Project)
Investigators: Kreyling, Wolfgang , Ziesenis, A. , Erbe, Franz , Oberdörster, Günter , Semmler, M. , Mayer, P. , Takenaka, S.
Institution: University of Rochester
Current Institution: GSF - Forschungszentrum fur Umwelt und Gesundheitand Ludwig Maximilian University , University of Rochester
EPA Project Officer: Chung, Serena
Project Period: June 1, 1999 through May 31, 2006
RFA: Airborne Particulate Matter (PM) Centers (1999) RFA Text | Recipients Lists
Research Category: Particulate Matter , Air Quality and Air Toxics , Air
Objective:
The objective of this research project is to test the hypothesis that ultrafine insoluble particles may translocate from deposition sites in the lungs to systemic circulation. If such translocation occurs, it could lead to accumulation and potentially adverse reactions in critical organs such as the liver, heart, and brain, consistent with epidemiological evidence that ultrafine insoluble particles may play a role in the onset of cardiovascular diseases.
Approach:
This research project will conduct inhalation experiments on healthy young male rats. In order to study ultrafine particle translocation into secondary organs, aerosols of ultrafine iridium particles radio-labeled with Ir-192 will be produced with a spark generator using neutron-activated pure iridium electrodes. For inhalation, WKY rats will be ventilated for one hour via an endotracheal tube. After exposure, rats will be monitored in metabolic cages and excreta will be collected separately and quantitatively. Rats also will be sacrificed in time intervals ranging from 6 hours to 21 days, so that researchers can conduct a complete analysis of Ir-92 activity retained in various organs, tissues, and the remaining carcass and cleared excreta.
Expected Results:
This study will analyze the fate of retained particles in the lungs versus that of translocated particles to either the gastrointestinal tract and feces or to other organs after systemic translocation. As a result of this study, we expect to be able to explain the proportion to which ultrafine particles translocate to the systemic circulation and extrapulmonary organs.
Supplemental Keywords:
ultrafine particles, iridium, I-92, cardiovascular diseases., RFA, Health, Air, Scientific Discipline, Health Risk Assessment, Risk Assessments, Air Pollution, particulate matter, Environmental Chemistry, Molecular Biology/Genetics, Air Pollution Effects, Atmospheric Sciences, Biochemistry, Toxicology, Air Pollutants, air toxics, aerosol, aerosols, health effects, sensitive populations, urban air pollution, human health, pathophysiological mechanisms, ambient monitoring, particle size, pulmonary disease, urban environment, coronary artery disease, fine particles, mortality, risk assessment, ambient air monitoring, iridium, cardiopulmonary responses, cardiovascular vulnerability, lung, morbidity, pulmonary, animal model, particulate exposure, environmental health effects, particulates, susceptible populations, ultrafine iridium particles, ambient air, biostatistics, inhalation toxicology, ambient air quality, cardiopulmonary, cardiovascular disease, human health effects, lung inflamation, atmospheric, ultrafine particlesProgress and Final Reports:
Main Center Abstract and Reports:
R827354 Health Effects Institute (2000 — 2005) Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R827354C001 Characterization of the Chemical Composition of Atmospheric Ultrafine Particles
R827354C002 Inflammatory Responses and Cardiovascular Risk Factors in Susceptible Populations
R827354C003 Clinical Studies of Ultrafine Particle Exposure in Susceptible Human Subjects
R827354C004 Animal Models: Dosimetry, and Pulmonary and Cardiovascular Events
R827354C005 Ultrafine Particle Cell Interactions: Molecular Mechanisms Leading to Altered Gene Expression
R827354C006 Development of an Electrodynamic Quadrupole Aerosol Concentrator
R827354C007 Kinetics of Clearance and Relocation of Insoluble Ultrafine Iridium Particles From the Rat Lung Epithelium to Extrapulmonary Organs and Tissues (Pilot Project)
R827354C008 Ultrafine Oil Aerosol Generation for Inhalation Studies
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.