Grantee Research Project Results
2020 Progress Report: Initial Annual CEMALB Progress Report: Human Health Effects of Environmental Pollutants
EPA Grant Number: CR835785Subproject: this is subproject number R835785 , established and managed by the Center Director under a main grant
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: Center for Environmental Medicine, Asthma, lung biology
Center Director: Peden, David B
Title: Initial Annual CEMALB Progress Report: Human Health Effects of Environmental Pollutants
Investigators: Peden, David B , Jaspers, Ilona , Hernandez, Michelle L
Current Investigators: Peden, David B , Jaspers, Ilona , Hernandez, Michelle L , Alexis, Neil , Caughey, Melissa , Rebuli, Meghan E
Institution: University of North Carolina at Chapel Hill
EPA Project Officer: Schmitt, Michael
Project Period: April 1, 2015 through March 31, 2022
Project Period Covered by this Report: April 1, 2020 through March 31,2021
Project Amount: $9,800,000
RFA: Human Health Effects of Environmental Pollutants (2014) RFA Text
Research Category: Human Health
Objective:
As originally proposed, this center is organized to be responsive to the research areas outlined in the funding announcement, which included: a) Multi-pollutant Interactions, b) Health Effects Associated with Inhaled Particulate Pollutants Derived from Specific Sources, c) Susceptibility to Air Pollutants, d) Social Determinants of Health, e) Intervention Studies, and f) Biological Mechanisms.
Progress Summary:
COVID Project 1: Effectiveness of UNC Hospital Facemask Alternatives for the COVID-19 Pandemic
Due to limited national supplies of N95 facemasks, UNC hospitals have accepted public and private company donations of personal protective equipment, including many types of face masks. With so many options, infection prevention experts at the UNC Medical Center asked CEMALB, in collaboration with the EPA HSF and TRC contractors, if they could measure the protectiveness of various types of masks and alternative options, including sterilized N95 masks, expired N95 masks, foreign-sourced masks, as well as surgical and homemade masks. To achieve these ends, an EPA exposure chamber was filled with airborne particles (50nm NaCl) to measure how many of those particles were able to penetrate each mask type fitted on an adult male and female subject, i.e. fitted filtration efficiency for each mask type.
To date we have found that gas sterilized (hydrogen peroxide and ethylene oxide) N95 masks have maintained their filtration capacity to greater than 98% efficiency, i.e. less than 2% penetration beyond the mask into the subjects breathing space. With regards to expired masks, these studies have also shown that 11-year-old expired 3M N95 masks were still greater than 95% effective. Future studies include continued efficiency assessment of new masks for both the UNC Medical Center and students/staff/faculty at UNC Chapel Hill.
COVID Project 2: Protocol 20-0937-ScreenNC: Screening seroprevalence of IgG antibody to SARS-CoV2 virus in asymptomatic UNC Health Patients
This is a SARS-CoV2 seroprevalence study supported by the UNC School of Medicine, UNC Health, NIEHS and this Cooperative Agreement. This study is a multi-site study in which persons asymptomatic for COVID-19 are recruited for screening for IgG antibody to the SARS-CoV2 virus. Volunteers must be UNC patients and test results are entered into the Epic UNC electronic health record data system, curated in the Carolina Data Warehouse for Health. Investigators have permission to analyze data from recruited volunteers as well as any UNC patients who have been tested for this antibody. Volunteers are recruited at the UNC Family Medicine Center, CTRC, the UNC Emergency Department, the Rex Emergency Department, Nash Medical Center, and UNC Physician Network. This catchment area (counties in which each recruitment site is located and adjoining counties) represents 44% of the regions in which SARS-CoV2 infection has been reported.
Recruitment began on 4/28/2020 and to date, data from 4,617 patients has been acquired, with data on 3,101 patients who were positive by viral swab also being available. Our initial report focuses on our initial 3,035 volunteers recruited into the study, to determine the seroprevalence of IgG to SARS-CoV2 in asymptomatic people. However, subsequent analyses and studies using this data set will focus on the impact of environmental factors (PM2.5, O3, SES factors, geocoded locations) on likelihood of being exposed and severity of disease in those who are seropositive.
Project 1: Development of Interventions to mitigate Inflammatory Response to Pollutants
The aims of Project 1 were modified in year 1 and outlined in previous years project report. As noted at the time of modification in year 1, the primary activities of this project are to: 1.) to identify characteristics of people at risk for adverse response to pollutants; 2.) develop specific interventions to mitigate the inflammatory response to pollutants; 3.) to develop personalized wearable sensors to allow individuals to understand their personal exposures and physiological state (e.g. lung function, pulse, accelerometry) in conjunction with the ASSIST center at NCSU: and 4.) to develop programs in environmental precision medicine and health using big data approaches (with funding of an above base supplement to address some aspects of this aim).
Project 2: Development of Methodologies to Assess Health Effects Associated with Pollutant Exposures
Oxidant pollutants derived from the incomplete combustion of organic matter, such as burning of biomass or woodsmoke, are of increased global public health concern and associated with increased susceptibility to respiratory infections, including viral infections in children. More than two billion people in the world use biomass (including indoor wood burning) as their main source of energy for domestic heating and cooking. Epidemiological evidence suggests that reducing exposure to biomass smoke exposure is associated with a decrease in upper and lower respiratory tract infections. Collaborative studies among investigators from UNC and EPA developed a human in vivo exposure and viral challenge study to translate these findings into human. These studies were built on human in vivo experimental models developed by investigators at UNC using the FDA-approved live attenuated influenza virus (LAIV) vaccine as a model for viral infections in human volunteers. Inoculation with LAIV causes a self-limiting replicative infection, which induces similar cytokine and antiviral host defense responses as community-acquired infections. This human LAIV inoculation model was previously used to assess interactions between exposure to diesel exhaust (DE) and viral infections, which demonstrated that underlying allergy rhinitis enhances the interaction between DE and viral infection, leading to increased viral replication and markers of allergic inflammation.
Project 3: Establish whether LPS inhalation induces Systemic inflammation and CV inflammation
The objective of Project 3 is to examine the role of biological components of PM on cardiovascular outcomes. To accomplish this overall objective, two protocols were developed using inhaled Clinical Center Reference Endotoxin as a model PM exposure.
The goal of this study (also known as “Endoscreen”) was to assess 18 healthy volunteers with an inhaled LPS challenge to first establish responsiveness to inhaled LPS. It is estimated that up to 30% of the population may not be responsive to inhaled LPS. Inhaled LPS response will be established by detecting at least a 20% increase from baseline levels of neutrophils in the blood, and/or by a 10% increase from baseline levels of neutrophils in induced sputum samples. In addition, as secondary endpoints, we evaluated inflammatory cytokine levels in systemic circulation and assessed for the presence of genes thought to be related to endotoxin response. Subjects wore a cardiac monitor known as Faros 180 to monitor heart rate variability, an autonomic index that has been previously shown to be affected by high dose ozone exposure (Devlin RB et al, Circulation, 2012). The Endoscreen study provided not only data on LPS responsiveness, but also provided preliminary associations between changes in LPS-induced heart rate variability.
Project 4: Above Base Project: Large Dataset Analyses to Determine Pollutant-induced Adverse Health Effects
By creating linkages of existing medical records and environmental datasets, we will investigate the impact of in utero and childhood exposure to air pollutants and wildfire smoke on childhood atopic and respiratory diseases including asthma, infant wheeze, etc. These investigations propose to use medical records data, such as those available through the prescription data from MarketScan and Medicare databases and North Carolina state birth registry data. For this project, the following goals have been accomplished:
- OA exposure inhibited relaxation of rings to the vasodilator acetylcholine (which acts via endothelial cell mechanisms). This inhibition of vasodilation is similar to the finding that exposure of volunteers to either diesel or biodiesel exhaust attenuated the response of endothelial cell mediated vasodilation.
- OA changed lung function parameters in rats in vivo, increased markers of inflammation and lung injury
- OA increase iron load in lung macrophages in vivo and co-administration of OA and ferric ammonium citrate (FAC) tended to increase the responses (neutrophilia, cytokines) relative to oleic acid alone
Project 5: Above Base Project: Autophagy as a Key Determinant of Cell Fate in Air Pollution Health Effects Among Healthy and Type 2 Diabetics
In pursuing the new focus, the involvement of immune cell changes and pulmonary inflammation were assessed at protein and gene expression levels in the lungs, BALF and serum samples from a study involving ozone exposure in rats with SHAM, adrenal demedullation or total bilateral adrenalectomy surgeries to examine the contribution of stress hormones. Additional assessment of samples involving lung tissue gene expression profiling in SHAM and adrenalectomized rats using global RNASeq is completed as the specific aim 1. Studies proposed for specific aim 2A involving pharmacological means of intervening stress hormone receptors have been completed. Aim 2B study, which will include adrenalectomy in conjunction with pharmacological interventions, has also been completed. Andres Henriquez successfully defended his doctoral dissertation and this work has already resulted in 7 publications with additional publications currently submitted/under review.
Project 6: Above Base Project: Environmental Health Education
For the spring 2020, our application and selection process for the David Holbrook travel award were disrupted by the COVID-19 related shutdown of UNC and cancellation of all national meetings in the spring.
A committee composed of current predoctoral and postdoctoral trainees associated with the Curriculum in Toxicology & Environmental Medicine generates a list of experts in environmental public health (usually 5-6) and ranks them based on interest of their research focus to the trainees in environmental health and track record in training and mentoring young scientists. The top candidate is invited for a seminar at UNC-CH and discussions with local scientists. The committee organizes the invitation, the seminar, as well as the overall itinerary for the invited speaker. The attendance of the seminar usually exceeds 50 attendees, including many investigators from CEMALB and EPA. During the current reporting period, the following investigator was chosen to the “Invited Environmental Public Health Seminar Speakers”. Dr. Lisa Miller Professor, University of California at Davis, Health Effects of Air Pollution on Susceptible Populations: Lessons Learned from Nonhuman Primates.
Project 7: Above Base Project: Efficacy of Fish Oil or Olive Oil Supplementation on the Health Effects of Ozone Exposure in Healthy Young Subjects (OMEGOZ) Project 7Above Base Project: Efficacy of Fish Oil or Olive Oil Supplementation on the Health Effects of Ozone Exposure in Healthy Young Subjects (OMEGOZ)
While working on Omegoz, Ms. Corteselli (student in Dr. Samet’s lab) optimized a protocol to analyze the bioenergetic profile of induced sputum samples using the Seahorse Extracellular Flux Analyzer. The analysis measures several aspects of both mitochondrial and glycolytic function. Following optimization, Ms. Corteselli processed the post-air and post-ozone exposure sputum samples (N= 4) collected from four Omegoz subjects. Seahorse analysis requires a minimum number of viable sputum cells for successful analysis. Sputum cell recovery has been variable to date with Omegoz and thus has presented a challenge with this part of the study.
Project 8: Above Base Project: The influence of stress, neighborhoods and the built environment on environmental health
Jennifer Styles, a graduate student with the Department of Environmental Sciences and Engineering, is currently conducting experiments assessing differences in the microbiome of community-based samples. Experimental parameters and protocols have been established, and data are being collected/analyzed. Specifically, since May 2019, the primary samples (air microbiome samples) for this project have been collected and analyzed. Jennifer Styled developed and defended her dissertation proposal in March 2020. During the current reporting period, this work has resulted in 2 manuscripts with 2 additional papers currently under review.
Project 9: Above Base Project: Effect of fuel and combustion conditions on pulmonary toxicity of smoke emissions
Dr. Yong Ho Kim, was hired as a Senior Research Associate for CEMALB working under the supervision of Dr. Jaspers. He has been instrumental in developing experimental models and combustion systems to generate samples mimicking the emissions from burning biomass fuel sources (e.g. wood) as well as waste disposed in military burn pits (e.g. plastics, plywood, carboard, etc.). These samples have been used for laboratory animal in vivo and human cell culture in vitro exposures to examine relative toxicities of these emission mixtures. Chemical analyses of these samples have been partially completed, which will be integrated with the biological toxicity matrix to rank the materials and resulting emission components for their ability to induce adverse health effects. Data from this project was used to support a newly funded NIEHS/NIH R21 application, which started 05/01/2020 with Dr. Kim as a collaborator on this project. In addition, one manuscript is currently being prepared summarizing the initial computational analysis identifying chemical classes in combustion emission mixtures driving biological responses.
Future Activities:
Project 1:
- ICEES can also be employed to assess pollutant impacts on clinical outcomes to achieve policy changes. An example of an immediate policy outcome from this project builds on ongoing collaborative environmental informatics research currently underway by CEMALB and EPA scientists exploring environmental impacts on cardiovascular disease using patient data from the CDWH. ICEES would allow these analyses to occur in a fashion in which personal identifiers are modified, so that primary data may be reviewed by federal reviewers in such a way that individual privacy is guaranteed. This approach has regulatory application for periodic assessment of the NAAQS of each pollutant mandated by the Clean Air Act.
- We will apply ICEES to explore environmental impacts on temporally discreet public health events. In addition to ScreenNC above, we anticipate undertaking an assessment of health outcomes in the UNC Health population due to PM2.5 increases caused by the 2008 NC Wildland Fires.
- We will use ICEES to explore the effect of the GSTM1 null genotype on asthma exacerbation and other clinical outcomes, guided by our published reports and leveraging our prior work.
- Note that the ICEES tool developed by this project and the subsequent use cases are also available to the studies outlined in Above Base Projects 4 and
Project 2:
Objective 1: Compare the effects of controlled woodsmoke exposures to other sources of combustion emissions, such as those encountered as part of military burn pit exposures. Initial in vitro studies comparing woodsmoke exposure to emissions generated by burning plastics, plywood, cardboard, and mixtures thereof are underway and biological responses and toxicities will be compared to those seen after woodsmoke exposures. Computational analyses will be used to identify potential classes of chemicals within woodsmoke or other combustion emission mixtures driving biological responses
Objective 2: Develop, optimize, and validate non-invasive, field-deployable sampling techniques, allowing investigators to sample the respiratory mucosa for pulmonary biomarkers, focus on implementing the sampling technique to other ongoing studies, and expand the scope of sample analyses. As described above, we have conducted initial pilot experiments to determine exposure-dependent changes in the nasal microbiome as well as chemical analyses, which could be used to determine exposures, including components and concentrations. Several studies led by Dr. Rebuli are implementing nasal ELF collection to determine biomarkers of disease and exposure in a variety of different cohorts. Objective 3 (New): Use non-invasive sampling techniques to identify biomarkers of SARS-CoV-2 infection. Our non-invasive nasal mucosal sampling technique is currently part of 4 ongoing COVID-19 cohort studies at UNC. These samples will be analyzed for viral load as well as nasal biomarkers of immune responses. Data will be stratified based on demographic information, potential co-exposures, and other risk factors
Project 3:
EndoScreen Study: Using the multiplex protein data from serum and sputum as well as mRNA data, we will identify mediators that are associated with an airway or systemic response to inhaled LPS challenge.
EndoHeart Study: In the upcoming months, we will finish analyses of the remaining CV data (BP, FMD, and LVS). We will also analyze sputum and serum mediators for inflammatory cytokines; specifically, we will focus on examining the role of LPS-induced IL-1β on airway and systemic cardiovascular responses on CV outcomes. This work will be prepared for publication
AsthmaSnoz: When the O3 chambers are permitted to reopen, we will first submit this project for IRB review to determine if it is permissible to continue this project in the setting of COVID-19 exposures since participants have asthma. If allowed to continue, we will attempt to re-recruit the 5 participants who completed the first exposure sessions and finish recruitment of remaining participants.
Project 4:
An IRB protocol to linking medical datasets and wildfire exposure data in North Carolina was just approved (UNC Study #20-1196) and analysis will start over the summer.
Project 5:
Work Complete
Project 6:
Requests for applications for this award will start in September, 2020 and complete applications need to be received by middle of October, 2020 to be eligible. Similarly, for spring meetings we will request applications in January 2021 and complete the review by February 2021. We anticipate 5-10 applications to make 2-3 awards.
Project 7:
This project is in progress and sputum samples continue to be processed in the Alexis lab for analysis of inflammatory endpoints. To date sputum samples from 40 subjects have been collected and processed. Differential cell counts have been performed on all samples to date. Supernatant samples are currently stored at -80 deg C for future fluid phase analysis. Elizabeth Cortiselli’s specific involvement however, with the Seahorse (extracellular flux/bioenergetics) analysis of the sputum samples has been discontinued and diverted to another project.
Project 9:
Based on the initial toxicity studies, experiments are ongoing further characterizing adverse biological effects induced by exposure to these different emission mixtures. Biological effects will be integrated with chemical characterizations to determine potential cause-and-effect relationships between emission components and toxicities. Furthermore, the experimental systems from this project are currently being expanded to develop additional exposure systems on the UNC campus and within the EPA.
Journal Articles: 50 Displayed | Download in RIS Format
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Bowers EC, McCullough SD. Linking the epigenome with exposure effects and susceptibility:The epigenetic seed and soil model. Toxicological Sciences 2016;155(2):302-14 |
CR835785 (2016) CR835785 (2017) CR835785 (2020) |
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Hickman E, Smyth T, Cabos-Uribe C, Immormino R, Rebuli M, Moran T, Alexis N, Jaspers I. Expanded characterization of in vitro polarized M0, M1, and M2 human monocyte-derived macrophages: Bioenergetic and secreted mediator profiles. PLOS ONE 2023;18(3):e0279037 |
CR835785 (Final) |
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Kobernick AK, Peden DB, Zhou H, Zhou Q, Dillon MA, Alexis NE. Reproducibility of the inflammatory response to inhaled endotoxin in healthy volunteers. Journal of Allergy and Clinical Immunology. 2016;138(4):1205-7 |
CR835785 (2015) CR835785 (2020) |
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Martin WK, Tennant AH, Conolly RB, Prince K, Stevens JS, DeMarini DM, Martin BL, Thompson LC, Gilmour MI, Cascio WE, Hays MD. High-throughput video processing of heart rate responses in multiple wild-type embryonic Zebrafish per imaging field. Scientific reports 2019;9(1):1-4. |
CR835785 (2019) CR835785 (2020) |
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McCullough SD, Bowers EC, On DM, Morgan DS, Dailey LA, Hines RN, Devlin RB, Diaz-Sanchez D. Baseline chromatin modification levels may predict interindividual variability in ozone-induced gene expression. Toxicological Sciences. 2015;150(1):216-24 |
CR835785 (2015) CR835785 (2017) CR835785 (2020) |
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McCullough SD, On DM, Bowers EC. Using Chromatin Immunoprecipitation in Toxicology:A Step‐by‐Step Guide to Increasing Efficiency, Reducing Variability, and Expanding Applications. Current protocols in toxicology 2017;72(1):3-14 |
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Miller DB, Snow SJ, Henriquez A, Schladweiler MC, Ledbetter AD, Richards JE, Andrews DL, Kodavanti UP. Systemic metabolic derangement, pulmonary effects, and insulin insufficiency following subchronic ozone exposure in rats. Toxicology and applied pharmacology 2016;306:47-57 |
CR835785 (2016) CR835785 (2017) CR835785 (2020) |
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Mirowsky JE, Peltier RE, Lippmann M, Thurston G, Chen LC, Neas L, Diaz-Sanchez D, Laumbach R, Carter JD, Gordon T. Repeated measures of inflammation, blood pressure, and heart rate variability associated with traffic exposures in healthy adults. Environmental Health. 2015;14(1):66 |
CR835785 (2015) CR835785 (2020) |
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Mirowsky JE, Devlin RB, Diaz-Sanchez D, Cascio W, Grabich SC, Haynes C, Blach C, Hauser ER, Shah S, Kraus W, Olden K. A novel approach for measuring residential socioeconomic factors associated with cardiovascular and metabolic health. Journal of Exposure Science and Environmental Epidemiology 2017;27(3):281 |
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Perryman A, Kim H, Payton A, Rager J, McNell E, Rebuli M, Wells H, Almond M, Antinori J, Alexis N, Porter N, Jasters I. Plasma sterols and vitamin D are correlates and predictors of ozone-induced inflammation in the lung: A pilot study. PLOS ONE 2023;18(5):e285721 |
CR835785 (Final) |
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Walsh DM, McCullough SD, Yourstone S, Jones SW, Cairns BA, Jones CD, Jaspers I, Diaz-Sanchez D. Alterations in airway microbiota in patients with PaO2/FiO2 ratio≤ 300 after burn and inhalation injury. PloS one 2017;12(3):e0173848 |
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Zetlen H, Cao K, Schichlein K, Knight N, Maecker H, Nadeau K, Rebuli M, Rise M. Comparison of multiplexed protein analysis platforms for the detection of biomarkers in the nasal epithelial lining fluid of healthy subjects. JOURNAL OF IMMUNOLOGICAL METHODS 2023;517(113473). |
CR835785 (Final) |
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Thurston GD, Balmes JR, Garcia E, Gilliland FD, Rice MB, Schikowski T, Van Winkle LS, Annesi-Maesano I, Burchard EG, Carlsten C, Harkema JR. Outdoor Air Pollution and New-Onset Airway Disease. An Official American Thoracic Society Workshop Report. Annals of the American Thoracic Society 2020;17(4):387-98. |
CR835785 (2019) CR835785 (2020) |
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Bass V. San Antonio Oleic acid induces acute pulmonary injury and inflammation in vivo. Society of Toxicology, 2017;10(1):34 |
CR835785 (2017) CR835785 (2020) |
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Miller DB, Karoly ED, Jones JC, Ward WO, Vallanat BD, Andrews DL, Schladweiler MC, Snow SJ, Bass VL, Richards JE, Ghio AJ. Inhaled ozone (O3)-induces changes in serum metabolomic and liver transcriptomic profiles in rats. Toxicology and applied pharmacology 2015;286(2):65-79. |
CR835785 (2017) CR835785 (2020) |
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Pawlak EA, Noah TL, Zhou H, Chehrazi C, Robinette C, Diaz-Sanchez D, Müller L, Jaspers I. Diesel exposure suppresses natural killer cell function and resolution of eosinophil inflammation:a randomized controlled trial of exposure in allergic rhinitics. Particle and fibre toxicology. 2015;13(1):24 |
CR835785 (2015) CR835785 (2020) |
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Cascio WE, Gilmour MI, Peden DB. Ambient air pollution and increases in blood pressure:role for biological constituents of particulate matter. Hypertension. 2015;66(3):469-71. |
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Mirowsky J, Gordon T. Noninvasive effects measurements for air pollution human studies:methods, analysis, and implications. Journal of Exposure Science and Environmental Epidemiology. 2015;25(4):354 |
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Rebuli ME, Speen AM, Clapp PW, Jaspers I. Novel applications for a noninvasive sampling method of the nasal mucosa. American Journal of Physiology-Lung Cellular and Molecular Physiology 2016;312(2):L288-96 |
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Duran CG, Burbank AJ, Mills KH, Duckworth HR, Aleman MM, Kesic MJ, Peden DB, Pan Y, Zhou H, Hernandez ML. A proof-of-concept clinical study examining the NRF2 activator sulforaphane against neutrophilic airway inflammation. Respiratory Research 2016;17(1):89. |
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Mirowsky JE, Dailey LA, Devlin RB. Differential expression of pro-inflammatory and oxidative stress mediators induced by nitrogen dioxide and ozone in primary human bronchial epithelial cells. Inhalation toxicology. 2016;28(8):374-82 |
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Snow SJ, Gordon CJ, Bass VL, Schladweiler MC, Ledbetter AD, Jarema KA, Phillips PM, Johnstone AF, Kodavanti UP. Age-related differences in pulmonary effects of acute and subchronic episodic ozone exposures in Brown Norway rats. Inhalation toxicology 2016;28(7):313-23. |
CR835785 (2017) CR835785 (2020) |
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Dieffenderfer J, Goodell H, Mills S, McKnight M, Yao S, Lin F, Beppler E, Bent B, Lee B, Misra V, Zhu Y. Low-power wearable systems for continuous monitoring of environment and health for chronic respiratory disease. IEEE journal of biomedical and health informatics. 2016;20(5):1251-64. |
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Henriquez A, House J, Miller DB, Snow SJ, Fisher A, Ren H, Schladweiler MC, Ledbetter AD, Wright F, Kodavanti UP. Adrenal-derived stress hormones modulate ozone-induced lung injury and inflammation. Toxicology and applied pharmacology 2017;329:249-58 |
CR835785 (2016) CR835785 (2017) CR835785 (2020) |
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Snow SJ, McGee MA, Henriquez A, Richards JE, Schladweiler MC, Ledbetter AD, Kodavanti UP. Respiratory effects and systemic stress response following acute acrolein inhalation in rats. Toxicological Sciences 2017;158(2):454-64 |
CR835785 (2016) CR835785 (2017) CR835785 (2020) |
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Miller CN, Dye JA, Ledbetter AD, Schladweiler MC, Richards JH, Snow SJ, Wood CE, Henriquez AR, Thompson LC, Farraj AK, Hazari MS. Uterine artery flow and offspring growth in long-evans rats following maternal exposure to ozone during implantation. Environmental health perspectives 2017;125(12):127005 |
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Mirowsky JE, Carraway MS, Dhingra R, Tong H, Neas L, Diaz-Sanchez D, Cascio W, Case M, Crooks J, Hauser ER, Dowdy ZE. Ozone exposure is associated with acute changes in inflammation, fibrinolysis, and endothelial cell function in coronary artery disease patients. Environmental Health 2017;16(1):126 |
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Zhou S, Behrooz L, Weitzman M, Pan G, Vilcassim R, Mirowsky JE, Breysee P, Rule A, Gordon T. Secondhand hookah smoke:an occupational hazard for hookah bar employees. Tobacco control. 2017;26(1):40-5 |
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Burbank AJ, Peden DB. Assessing the impact of air pollution on childhood asthma morbidity:how, when, and what to do. Current opinion in allergy and clinical immunology2018;18(2):124-31 |
CR835785 (2018) CR835785 (2020) |
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Henriquez AR, Snow SJ, Schladweiler MC, Miller CN, Dye JA, Ledbetter AD, Richards JE, Mauge-Lewis K, McGee MA, Kodavanti UP. Adrenergic and glucocorticoid receptor antagonists reduce ozone-induced lung injury and inflammation. Toxicology and applied pharmacology 2018;339:161-71 |
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Snow SJ, Henriquez AR, Costa DL, Kodavanti UP. Neuroendocrine regulation of air pollution health effects:emerging insights. Toxicological Sciences 2018;164(1):9-20 |
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Huang MC, Douillet C, Dover EN, Stýblo M. Prenatal arsenic exposure and dietary folate and methylcobalamin supplementation alter the metabolic phenotype of C57BL/6J mice in a sex-specific manner. Archives of toxicology 2018;92(6):1925-37 |
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Hazari MS, Stratford KM, Krantz QT, King C, Krug J, Farraj AK, Gilmour MI. Comparative cardiopulmonary effects of particulate matter-and ozone-enhanced smog atmospheres in mice. Environmental science & technology 2018;52(5):3071-80. |
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Stratford K, Haykal-Coates N, Thompson L, Krantz QT, King C, Krug J, Gilmour MI, Farraj A, Hazari M. Early-life persistent vitamin D deficiency alters cardiopulmonary responses to particulate matter-enhanced atmospheric smog in adult mice. Environmental science & technology 2018;52(5):3054-61. |
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Snow SJ, Cheng WY, Henriquez A, Hodge M, Bass V, Nelson GM, Carswell G, Richards JE, Schladweiler MC, Ledbetter AD, Chorley B. Ozone-induced vascular contractility and pulmonary injury are differentially impacted by diets enriched with coconut oil, fish oil, and olive oil. Toxicological Sciences 2018;163(1):57-69 |
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Henriquez AR, Snow SJ, Schladweiler MC, Miller CN, Dye JA, Ledbetter AD, Richards JE, Hargrove MM, Williams WC, Kodavanti UP. Beta-2 adrenergic and glucocorticoid receptor agonists modulate ozone-induced pulmonary protein leakage and inflammation in healthy and adrenalectomized rats. Toxicological Sciences 2018;166(2):288-305. |
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Egorov AI, Converse R, Griffin SM, Styles J, Klein E, Sams E, Hudgens E, Wade TJ. Environmental risk factors for Toxoplasma gondii infections and the impact of latent infections on allostatic load in residents of Central North Carolina. BMC infectious diseases 2018;18(1):421. |
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Lyerly HK, Peden DB. Health and the Environment in North Carolina. North Carolina medical journal2018;79(5):302-5 |
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Peden DB. The Unexpected Health Effects of Air Pollution. North Carolina medical journal2018;79(5):309-11 |
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Brooks JL, Berry DC, Currin EG, Ledford A, Knafl GJ, Fredrickson BL, Beeber LS, HAPPI Community Partnership Committee, Peden DB, Corbie‐Smith GM. A community‐engaged approach to investigate cardiovascular‐associated inflammation among American Indian women:A research protocol. Research in nursing & health201942(3):165-75 |
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Fecho K, Pfaff E, Xu H, Champion J, Cox S, Stillwell L, Peden DB, Bizon C, Krishnamurthy A, Tropsha A, Ahalt SC. A novel approach for exposing and sharing clinical data:the Translator Integrated Clinical and Environmental Exposures Service. Journal of the American Medical Informatics Association 2019;26(10):1064-1073 |
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Bass VL, Snow S, Soukup J, Schladweiler M, Ghio A, Kodavanti U, Madden MC. 12-hydroxy oleic acid impairs endothelium-dependent vasorelaxation. Journal of Toxicology and Environmental Health, Part A 2019;82(5):383-6. |
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Cromar KR, Duncan BN, Bartonova A, Benedict K, Brauer M, Habre R, Hagler GS, Haynes JA, Khan S, Kilaru V, Liu Y. Air Pollution Monitoring for Health Research and Patient Care. An Official American Thoracic Society Workshop Report. Annals of the American Thoracic Society 2019;16(10):1207-14. |
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Miller CN, Kodavanti UP, Stewart EJ, Schaldweiler M, Richards JH, Ledbetter AD, Jarrell LT, Snow SJ, Henriquez AR, Farraj AK, Dye JA. Aspirin pre-treatment modulates ozone-induced fetal growth restriction and alterations in uterine blood flow in rats. Reproductive Toxicology 2019;83:63-72. |
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Stevens EL, Rosser F, Forno E, Peden D, Celedón JC. Can the effects of outdoor air pollution on asthma be mitigated?. Journal of Allergy and Clinical Immunology 2019;143(6):2016. |
CR835785 (2019) CR835785 (2020) |
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Corteselli EM, Gibbs-Flournoy E, Simmons SO, Bromberg P, Gold A, Samet JM. Long chain lipid hydroperoxides increase the glutathione redox potential through glutathione peroxidase 4. Biochimica et Biophysica Acta (BBA)-General Subjects 2019;1863(5):950-9. |
CR835785 (2019) CR835785 (2020) |
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Zhang XA, Yates A, Vasilevsky N, Gourdine JP, Callahan TJ, Carmody LC, Danis D, Joachimiak MP, Ravanmehr V, Pfaff ER, Champion J. Semantic integration of clinical laboratory tests from electronic health records for deep phenotyping and biomarker discovery. NPJ digital medicine 2019;2(1):1-9. |
CR835785 (2019) CR835785 (2020) |
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Hargrove MM, Kim YH, King C, Wood CE, Gilmour MI, Dye JA, Gavett SH. Smoldering and flaming biomass wood smoke inhibit respiratory responses in mice. Inhalation Toxicology 2019;31(6):236-47. |
CR835785 (2019) CR835785 (2020) |
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Sood AK, Burbank AJ, Lawson M, Zhou H, Wells HB, Peden DB, Hernandez ML. Systemic inflammatory response to inhaled endotoxin does not correlate with airway response. Respiratory research 2019;20(1):1-4. |
CR835785 (2019) CR835785 (2020) |
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Kim YH, King C, Krantz T, Hargrove MM, George IJ, McGee J, Copeland L, Hays MD, Landis MS, Higuchi M, Gavett SH. The role of fuel type and combustion phase on the toxicity of biomass smoke following inhalation exposure in mice. Archives of toxicology 2019;93(6):1501-13. |
CR835785 (2019) CR835785 (2020) |
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Progress and Final Reports:
Original AbstractThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.
Project Research Results
- Final Report
- 2019 Progress Report
- 2018 Progress Report
- 2017 Progress Report
- 2016 Progress Report
- 2015 Progress Report
- Original Abstract
50 journal articles for this center