Grantee Research Project Results

2013 Progress Report: Predicting Food Protein Allergenicity Using a Mouse Model

EPA Grant Number: R834822
Title: Predicting Food Protein Allergenicity Using a Mouse Model
Investigators: Gangur, Venugopal
Institution: Michigan State University
EPA Project Officer: Aja, Hayley
Project Period: September 15, 2010 through September 14, 2014
Project Period Covered by this Report: September 15, 2012 through September 14,2013
Project Amount: $424,919
RFA: Approaches to Assessing Potential Food Allergy from Genetically Engineered Plants (2009) RFA Text | Recipients Lists
Research Category: Human Health

Objective:

1) Determine the NOAEL and the LOAEL of food proteins with high vs. low/no allergenic potential in a mouse model.

2) Determine the immune, clinical and physiological responses to isolated pure food proteins with high vs. low/no allergenic potential in this model.

Progress Summary:

(1) Determination of allergenicity and oral elicitation threshold doses (LOAEL and NOAEL) for hazelnut protein prepared by our laboratory;

(2) Evaluation of the allergenicity of sesame seed protein from two sources (our lab prepared sesame seed protein and obtained it from the Greer Lab) in the TS/OE model;

(3) Determination of oral elicitation threshold doses (LOAEL and NOAEL) for these two sources of sesame seed protein in TS/OE model; and

(4) Estimation of human equivalent threshold doses for sesame proteins from both sources.

Future Activities:

We plan to evaluate the allergenicity of purified protein ovalbumin, establish oral-dose response curves and percent responder curves, and estimate oral elicitation threshold doses (LOAEL and NOAEL) in TS/OE mouse model. Based on the mouse model data and using published methods, we will estimate the human equivalent threshold doses for ovalbumin. Subject to availability of adequate funds, we plan to evaluate allergenicity of another purified protein, rubisco, in this mouse model. We plan to submit a manuscript for publication and present an abstract for presentation during the American Association of Immunologists Annual Meeting to be held in May 2014.

Journal Articles:

No journal articles submitted with this report: View all 19 publications for this project

Supplemental Keywords:

food allergy, food safety, allergen, systemic anaphylaxis, exposure, hazard assessment, mouse model, threshold elicitation dose, NOAEL, LOAEL, potency testing, dose-response, genetic engineering, allergenicity

Relevant Websites:

http://www.hesiglobal.org/files/19-Gangur-Mouse%20Model-Hazelnut-May%202013.pdf Exit

Progress and Final Reports:

Original Abstract

The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.