Grantee Research Project Results
2010 Progress Report: Ultrafine Particle Cell Interactions In Vitro: Molecular Mechanisms Leading To Altered Gene Expression in Relation to Particle Composition
EPA Grant Number: R832415C005Subproject: this is subproject number 005 , established and managed by the Center Director under grant R832415
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: Rochester PM Center
Center Director: Oberdörster, Günter
Title: Ultrafine Particle Cell Interactions In Vitro: Molecular Mechanisms Leading To Altered Gene Expression in Relation to Particle Composition
Investigators: Finkelstein, Jacob N. , Prather, Kimberly A. , Rahman, Arshad , Oakes, David , Phipps, Richard
Institution: University of Rochester , University of California - San Diego
EPA Project Officer: Chung, Serena
Project Period: October 1, 2005 through September 30, 2010 (Extended to September 30, 2012)
Project Period Covered by this Report: June 30, 2009 through July 1,2010
RFA: Particulate Matter Research Centers (2004) RFA Text | Recipients Lists
Research Category: Human Health , Air
Objective:
Progress Summary:
Responses to particles by epithelial/endothelial co-cultures
As more of the physiological effects noted in our in vivo studies have pointed towards cardiovascular endpoints our emphasis on cellular models has shifted to vascular cell populations. The main focus of our in vitro studies continues to be the endothelium but while continuing to test the validity of the cellular mechanisms of response in different cell types. As a means of better correlating the potential of various particles or other stimuli to induce effects in vivo and establish appropriate in vitro models we continue to examine multiple cellular endpoints, in particular those that are relevant to measurement made in other PM center projects. Our results have established that measurement of IL 6 has the best predictive value. Once we have established the appropriate end points our plans have been to create more relevant models. Co-culture systems consisting of lung epithelial cells and lung endothelial cells have established so that both PM-cell and cell-cell interactions can be evaluated. Cell type specific responses of cytokine induction (TNF-á; IL-6; IL-8; or homologue) and NO-production (endothelial cells) will be measured as well as uptake and PM size-specific translocation. Dose-response relationships using PM samples collected at different sites from different sources will first be established in epithelial and endothelial cell monocultures. These data will be used to select the most active fractions of the PM samples for the co-culture studies and for the in vivo instillation studies.
Future Activities:
Journal Articles on this Report : 1 Displayed | Download in RIS Format
Other subproject views: | All 13 publications | 11 publications in selected types | All 10 journal articles |
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Other center views: | All 191 publications | 157 publications in selected types | All 144 journal articles |
Type | Citation | ||
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Rushton EK, Jiang J, Leonard SS, Eberly S, Castranova V, Biswas P, Elder A, Han X, Gelein R, Finkelstein J, Oberdorster G. Concept of assessing nanoparticle hazards considering nanoparticle dosemetric and chemical/biological response metrics. Journal of Toxicology and Environmental Health, Part A 2010;73(5-6):445-461. |
R832415 (2010) R832415 (2011) R832415 (Final) R832415C004 (2010) R832415C004 (2011) R832415C005 (2010) R832415C005 (2011) |
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Supplemental Keywords:
RFA, Scientific Discipline, Health, PHYSICAL ASPECTS, Air, particulate matter, Genetics, Health Risk Assessment, Risk Assessments, Physical Processes, Biology, altered gene expression, atmospheric particulate matter, long term exposure, atmospheric particles, airway disease, exposure, ambient particle health effects, human exposure, atmospheric aerosol particles, PM, aersol particlesProgress and Final Reports:
Original AbstractMain Center Abstract and Reports:
R832415 Rochester PM Center Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R832415C001 Characterization and Source Apportionment
R832415C002 Epidemiological Studies on Extra Pulmonary Effects of Fresh and Aged Urban Aerosols from Different Sources
R832415C003 Human Clinical Studies of Concentrated Ambient Ultrafine and Fine Particles
R832415C004 Animal models: Cardiovascular Disease, CNS Injury and Ultrafine Particle Biokinetics
R832415C005 Ultrafine Particle Cell Interactions In Vitro: Molecular Mechanisms Leading To Altered Gene Expression in Relation to Particle Composition
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.
Project Research Results
- Final Report
- 2011 Progress Report
- 2009 Progress Report
- 2008 Progress Report
- 2007 Progress Report
- 2006 Progress Report
- Original Abstract
10 journal articles for this subproject
Main Center: R832415
191 publications for this center
144 journal articles for this center