Grantee Research Project Results

2018 Progress Report: Systems Engineering & Analysis for Organotypic Culture Models

EPA Grant Number: R835736C005
Subproject: this is subproject number 005 , established and managed by the Center Director under grant R835736
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: Mickey Leland National Urban Air Toxics Research Center (NUATRC)
Center Director: Beskid, Craig
Title: Systems Engineering & Analysis for Organotypic Culture Models
Investigators: Wikswo, John , Cliffel, David , McLean, John , Shotwell, Matt
Institution: Vanderbilt University
EPA Project Officer: Aja, Hayley
Project Period: December 1, 2014 through November 30, 2018 (Extended to November 30, 2019)
Project Period Covered by this Report: December 1, 2017 through November 30,2018
RFA: Organotypic Culture Models for Predictive Toxicology Center (2013) RFA Text | Recipients Lists
Research Category: Chemical Safety for Sustainability

Objective:

VPROMPT's Project 5 is designed to ensure physiologically realistic function of each of the mammary, limb, fetal membrane, and liver organotypic culture models (OCMs), operating separately and in combination, and will do so by coordinating the refinement of Integrated Organ Microfluidics (IOM) modules that will provide a common platform architecture.

Progress Summary:

Project 5 continues to deliver standalone pumps, valves, IOM Perfusion Controllers (IOM-PCs, v2.0), and MicroClinical Analyzers (µCAs, v2.0) as requested by organ developers. A variety of Rotary Planar Peristaltic Micropumps (RPPMs) are in regular use by Projects 1 and 2, and we anticipate their use by Project 3 soon. Project 1 has evaluated the 5-port Rotary Planar Valves (RPVs). Project 5 has made significant progress in developing multi-port RPPMs and RPVs that will soon bring new capabilities to the pumps and valves used by all groups to perfuse OCMs and reduce the cost and increase the ease of use of the hardware. We designed, fabricated, and tested 8-port RPPMs and 2x8-port RPVs, and 6-port versions are being produced for the Project 1 mammary gland OCMs. VPROMPT is benefiting directly from a separately funded development of a new V3.0 motor cartridge, with circular through-plate fluidics, and an openable Puck OCM. The production of IOM fluidic chips has been streamlined with a new, soft-lithography molding process. The V3.0 IOM through-plate fluidic architecture has enabled the development of new and improved IOM fluidic chips, including Ridge, Capacitor, Push-Pull, and LoFlow RPPMs. Significant progress has been made towards full wireless control of both a Triple NEMA-8 IOM PC and a single V3.1 SmartMotor IOM Module, and the latter will be made available to VPROMPT in Q3 2019. We also have worked closely with Project 2 to develop an automated bone-construct limb-segmentation bender.

Journal Articles on this Report : 2 Displayed | Download in RIS Format

Publications Views
Other subproject views:	All 38 publications	8 publications in selected types	All 8 journal articles
Other center views:	All 169 publications	57 publications in selected types	All 56 journal articles

Publications
Type	Citation	Sub Project	Document Sources
Journal Article	Auner AW, Tasneem KM, Markov DA, McCawley LJ, Hutson MS. Chemical-PDMS Binding Kinetics and Implications for Bioavailability in microfluidic Devices. Lab on a Chip 2019;19(5):864-874	R835736C001 (2018) R835736C001 (Final) R835736C005 (2018)	Abstract from PubMed Abstract: Lab on a Chip - Abstract HTML Exit
Journal Article	Miller DR, McClain ES, Cliffel DE. Electrochemical Microphysiometry Detects Cellular Glutamate Up-take. Journal of The Electrochemical Society 2018;165(12):G3120-G3124.	R835736 (Final) R835736C005 (2018) R835736C005 (Final)	Full-text: Journal of The Electrochemical Society - Full Text HTML Exit Abstract: Journal of the Electrochemical Society - Abstract Exit Other: Journal of The Electrochemical Society - Full Text PDF Exit

Supplemental Keywords:

Microfluidic pumps and valves, integrated organ microfluidics

Relevant Websites:

Vanderbilt Institute for Integrative Biosystems Research and Education Exit

Vanderbilt-Pittsburgh Resource for Organotypic Models for Predictive Toxicology (VPROMPT) Exit

Progress and Final Reports:

Original Abstract

Main Center Abstract and Reports:

R835736 Mickey Leland National Urban Air Toxics Research Center (NUATRC)

Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R835736C001 Mammosphere Bioreactor For Life-Stage Specific Toxicology
R835736C002 Organotypic Culture Model to Analyze DevelopmentalLimbMalformationsResulting from Toxicant/Teratogen Exposure
R835736C003 Validating a fetal membrane on a chip model for characterizing reproductive toxicant exposure risks
R835736C004 Organotypic Liver Model for Predictive Human Toxicology and Metabolism
R835736C005 Systems Engineering & Analysis for Organotypic Culture Models

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The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.