Main Title |
Virion Aggregation and Disinfection of Water Viruses by Bromine. |
Author |
Sharp., D. Gordon ;
|
CORP Author |
North Carolina Univ. at Chapel Hill. Dept. of Bacteriology.;Municipal Environmental Research Lab., Cincinnati, Ohio. Water Supply Research Div. |
Year Published |
1976 |
Report Number |
EPA-R-802946; EPA/600/2-76-163; |
Stock Number |
PB-253 087 |
Additional Subjects |
Viruses ;
Bromine ;
Water treatment ;
Disinfection ;
Polioviruses ;
Reoviruses ;
Reaction kinetics ;
Agglomeration ;
Electron microscopy ;
Laboratory equipment ;
Concentration(Composition) ;
Water pollution ;
Centrifuging ;
Design criteria ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB-253 087 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
45p |
Abstract |
There have been many papers written about the survival of virus during exposure to disinfecting agents and most of them include, in the discussion of their results, some speculation regarding the possible influence of virus particle aggregation. This paper differs from the others. It provides direct evidence of the kind and degree of aggregation that was present among the virions that were treated with bromine as well as the PFU titers of the starters and the survivors. A dynamic system, in which disinfectant exposure time intervals as low as 0.5 seconds was developed. Polio and reovirus preparations, containing essentially all single particles, as indicated by electron microscopy, were tested in this system. The results indicated that reovirus single particle suspensions were 30 times as sensitive to bromine as poliovirus single particle suspensions, both showing essentially first order reaction kinetics with disinfection rates of 3 log base 10 per second and 6 log base 10 units per minute, respectively. Inactivation rates with slightly aggregated reovirus deviated from first order kinetics, the reaction rate declining continuously in a manner consistent with the degree of aggregation shown by electron micrographs. Temperature effects on disinfection rates using dispersed poliovirus were also investigated. The report demonstrates the effects of virus aggregation on disinfection kinetics and provides the basis for comparing the resistance of different viruses to different disinfectants under various conditions. This research can be applied to water pollution areas. |
NTIS Title Notes |
Final rept. Apr 74-May 75. |
PUB Date Free Form |
May 76 |
Category Codes |
06M; 57K; 68D |
NTIS Prices |
PC A03/MF A01 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
326618593 |
Origin |
NTIS |
Type |
CAT |