| Main Title |
Dynamics of Plasmid Transfer on Surfaces. |
| Author |
Simonsen, L. ;
|
| CORP Author |
Massachusetts Univ., Amherst. Dept. of Zoology.;Environmental Research Lab., Gulf Breeze, FL.;National Institutes of Health, Bethesda, MD. |
| Publisher |
c1990 |
| Year Published |
1990 |
| Report Number |
NIH-GM33782; EPA/600/J-90/367; |
| Stock Number |
PB91-163816 |
| Additional Subjects |
Plasmids ;
Escherichia coli ;
Cultured cells ;
Mutation ;
Kinetics ;
Growth ;
Reprints ;
Surface slide system
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB91-163816 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
9p |
| Abstract |
A protocol was developed to study the dynamics of growth and plasmid transfer in surface populations of bacteria. The method allows for quantitative estimates of cell population densities over time, as well as microscopic observations of colony growth and interactions. Using the 'surface slide system' (SSS), the dynamics of the plasmid R1 and its permanently derepressed mutant R1drd19 in surface cultures of Escherichia coli K12 was examined. A hypothesis for plasmid transfer between colonies that explains these observations as a consequence of the geometry of the surface habitat and the effect of transitory derepression of the synthesis of pili is proposed. |
| Supplementary Notes |
Pub. in Jnl. of General Microbiology 136, p1001-1007 1990. Sponsored by Environmental Research Lab., Gulf Breeze, FL., and National Institutes of Health, Bethesda, MD. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Dynamics of Plasmid Transfer on Surfaces. |
| Category Codes |
57F; 57K |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/04 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
113027634 |
| Origin |
NTIS |
| Type |
CAT |
