Main Title |
Immunoassay for p-Nitrophenol in Urine (Chapter XX). |
Author |
Rogers, K. R. ;
Van Emon, J. M. ;
|
CORP Author |
Environmental Monitoring Systems Lab., Las Vegas, NV. |
Publisher |
1993 |
Year Published |
1993 |
Report Number |
EPA/600/A-93/074; |
Stock Number |
PB93-175552 |
Additional Subjects |
Nitrophenols ;
Urinalysis ;
Immunoassay ;
Enzyme-linked immunosorbent assay ;
Occupational exposure ;
Organophosphate insecticides ;
Biological markers ;
Fluorescence spectometry ;
Reprints ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB93-175552 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
12p |
Abstract |
Urinary excretion of nitrophenol metabolites is an important index of human exposure to organophosphate pesticides. In particular, p-nitrophenol, a major urinary metabolite of parathion, can be used as a biomarker of human exposure. Immunoassay methods have been recently described for detection of p-nitrophenol. In the present paper, the effects of a urine matrix on the detection of p-nitrophenol are reported using both fluorescence and absorbance-based ELISAs. A competition format using the enzyme-amplified catalysis of 4-nitrophenyl phosphate or 4-methylumbelliferyl phosphate was used to report the analyte concentration. The presence of urine during the antibody-analyte interaction inhibited product formation in the final step of the assay and shifted the inhibition curves to the right, increasing the apparent I50 values for p-nitrophenol. The various urine samples collected from volunteers, not occupationally exposed to parathion, varied in their ability to inhibit color information and increase I50 values. |
Title Annotations |
Reprint: Immunoassay for p-Nitrophenol in Urine (Chapter XX). |
Category Codes |
57D; 57Y; 57U; 68E |
NTIS Prices |
PC A03/MF A01 |
Primary Description |
600/07 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
316130673 |
Origin |
NTIS |
Type |
CAT |