||Effects of Trichloroethylene and Its Metabolites on Rodent Hepatocyte Intercellular Communication.
Klaunig, J. E. ;
Ruch, R. J. ;
Lin, E. L. C. ;
||Medical Coll. of Ohio at Toledo.;Health Effects Research Lab., Cincinnati, OH.
Liver neoplasms ;
Dose-response relationships ;
Chronic exposure ;
Trichloracetic acid ;
Chloral hydrate ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||Chronic exposure to trichloroethylene (TCE) results in hepatocellular cancer in mice but not rats. The induction of hepatic tumors by TCE appears to be mediated through nongenotoxic or tumor promotion mechanisms. One cellular effect exhibited by a number of nongenotoxic carcinogens and tumor promoters is the inhibition of gap junction mediated intercellular communication. In the present study, the effects of trichloroethylene (TCE) and its metabolites, trichloracetic acid (TCA), trichloroethanol (TCEth), and chloral hydrate (CH) on gap junction mediated intercellular communication in cultured B6C3F1 mouse and F344 rat hepatocytes were assessed. TCE and TCA inhibited intercellular communication in mouse hepatocytes but not in rat hepatocytes. TCEth and CH had no effect on hepatocyte intercellular communication in either rat or mouse cells. TCE and TCA inhibited intercellular communication in both 24-hr-old and freshly plated mouse hepatocytes. Both compounds produced greater inhibition of intercellular communication in freshly plated cells when compared to 24-hr-old cultures. TCE appeared to require cytochrome P450 metabolism by the mouse hepatocytes to exhibit its inhibitory effect on dye coupling since treatment with SKF-525A prevented the inhibition of intercellular communication by TCE. (Copyright (c) 1989 by Academic Press, Inc.)
||Pub. in Toxicology and Applied Pharmacology, v99 n3 p454-465 Jul 89, Sponsored by Health Effects Research Lab., Cincinnati, OH.
|NTIS Title Notes
||Reprint: Effects of Trichloroethylene and Its Metabolites on Rodent Hepatocyte Intercellular Communication.
||57Y; 57E; 57I; 68G
||PC A03/MF A01