Main Title |
Quantitative Analysis of the Metabolism of Benzo(a)pyrene by Transformable C3H10T1/2CL8 Mouse Embryo Fibroblasts. |
Author |
Rudo, K. ;
Ellis, S. ;
Bryant, B. J. ;
Lawrence, K. ;
Curtis, G. ;
|
CORP Author |
Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. |
Year Published |
1986 |
Report Number |
EPA/600/J-86/221; |
Stock Number |
PB87-170346 |
Additional Subjects |
Pyrenes ;
Aromatic polycyclic hydrocarbons ;
Metabolism ;
Embryos ;
Mice ;
Laboratory animals ;
Cell morphology ;
Transformations ;
Toxicity ;
Carcinogens ;
Mutagens ;
Quantitative analysis ;
Reprints ;
Benzopyrenes ;
Fibroblasts ;
Metabolites
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB87-170346 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
15p |
Abstract |
The metabolism of benzo(a)pyrene (B(a)P) to organic-soluble and water-soluble metabolites by transformable C3H10T1/2CL8 mouse embryo fibroblasts was studied as a function of time, B(a)P concentration, and cell density. The total formation of organic-soluble and water-soluble metabolites increased with incubation time from 4 to 48 h and with B(a)P concentration from 4 to 40 micromolarity. As cell density increased, the metabolic rate decreased for organic-soluble and water-soluble products between 6,300 and 54,00 cells/sq cm probably due to decreases in B(a)P concentrations to values below saturation. Specific organic-soluble metabolites identified were B(a)P-pre-9,10-diol, B(a)P-7,8-diol, B(a)P-3,6-quinone, B(a)P-3-phenol, and B(a)P-9-phenol. Water-soluble metabolites were subjected to enzymatic hydrolysis with beta-glucuronidase and aryl sulfatase to identify specific conjugated products. The sulfate conjugated metabolites identified were B(a)P-7,8-diol, B(a)P-pre-9,10-diols, B(a)P-9,10-diol, and B(a)P-3,6-quinone. The beta-glucuronic acid metabolites identified were Ba(a)P-pre-9,10-diols, B(a)P-3,6-quinone, and B(a)P-3-phenol. Patterns of metabolite formation rates are discussed as to their possible effect on morphological transformation rates in C3H10T1/2 cells with respect to incubation time and cell density. (Copyright (c) 1986 Alan R. Liss, Inc.) |
Supplementary Notes |
Pub. in Teratogenesis, Carcinogenesis, and Mutagenesis 6, p307-319 1986. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Quantitative Analysis of the Metabolism of Benzo(a)pyrene by Transformable C3H10T1/2CL8 Mouse Embryo Fibroblasts. |
PUB Date Free Form |
c1986 |
Category Codes |
57Y; 57Z |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
716918501 |
Origin |
NTIS |
Type |
CAT |