Main Title |
Restriction Map of 'Rachiplusia ou' and 'Rachiplusia ou-Autographa californica' Baculovirus Recombinants. |
Author |
Smith, Gale E. ;
Summers, Max D. ;
|
CORP Author |
Texas A and M Univ., College Station. Dept. of Entomology.;Health Effects Research Lab., Research Triangle Park, NC. |
Year Published |
1980 |
Report Number |
EPA-R-805232-01; EPA-600/J-80-253; |
Stock Number |
PB81-192056 |
Additional Subjects |
Viruses ;
Deoxyribonucleic acid ;
Genes ;
Molecular biology ;
Microorganisms ;
Chromosomes ;
Cells(Biology) ;
Peptides ;
Reprints ;
Autographa californica ;
Rachiplusia ou
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB81-192056 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
11p |
Abstract |
The restriction sites of Rachiplusia ou nuclear polyhedrosis virus (RoMNPV) DNA were mapped for the endonucleases SmaI, KpnI, BamHI, SacI, XhoI, and EcoRI. Of the 60 DNA restriction sites of RoMNPV, 35 mapped in similar positions as compared to the restriction sites of Autographa californica nuclear polyhedrosis virus (AcMNPV) DNA. Two plaque-purified viruses, obtained from randomly picked plaques of a wild-type isolate of RoMNPV, were recombinants of RoMNPV and AcMNPV. The recombinants were shown to have RoMNPV and AcMNPV restriction fragments as well as structural polypeptides from each parental virus. Both recombinant viruses had a major RoMNPV capsid protein but were occluded in the AcMNPV polyhedrin protein. |
Supplementary Notes |
Pub. in Jnl. of Virology, v33 n1 p311-319 Jan 80. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Restriction Map of 'Rachiplusia ou' and 'Rachiplusia ou-Autographa californica' Baculovirus Recombinants. |
PUB Date Free Form |
1980 |
Category Codes |
6M; 6C; 57K; 57F |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
323797236 |
Origin |
NTIS |
Type |
CAT |