Main Title |
Method 1604 / total coliforms and Escherichia coli in water by membrane filtration using simultaneous detection technique (MI Medium). |
CORP Author |
Environmental Protection Agency, Washington, DC. Office of Water. |
Publisher |
Environmental Protection Agency, Office of Water, |
Year Published |
2002 |
Report Number |
EPA 821-R-02-024 |
Stock Number |
PB2003-100129 |
OCLC Number |
922532502 |
Subjects |
Escherichia coli--United States--Prevention ;
Enterobacteriaceae--United States
|
Additional Subjects |
Coliforms ;
Escherichia coli ;
Bacteria ;
Water sampling ;
Membrane filtration ;
Enumeration ;
Drinking water ;
Detection ;
Feces ;
Water pollution effects ;
Water quality ;
Contamination ;
Calibration ;
Pollution prevention ;
Microbial analysis ;
Quality control ;
Laboratories ;
Test methods
|
Internet Access |
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
ELBD ARCHIVE |
EPA 821-R-02-024 |
Received from HQ |
AWBERC Library/Cincinnati,OH |
10/04/2023 |
NTIS |
PB2003-100129 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
14 pages : illustrations ; 28 cm |
Abstract |
This test method describes a sensitive and differential membrane filter (MF) medium, using MI agar or MI broth, for the simultaneous detection and enumeration of both total coliforms (TC) and Escherichia coli (E. coli) in water samples in 24 hours or less on the basis of their specific enzyme activities. Two enzyme substrates, the fluorogen 4-Methylumbelliferyl- b-D-galactopyranoside (MUGal) and a chromogen Indoxyl-b-D-glucuronide (IBDG), are included in the medium to detect theenzymes b-galactosidase and b-glucuronidase, respectively, produced by TC and E. coli, respectively. Total coliforms include species that may inhabit the intestines of warm-blooded animals or occur naturally in soil, vegetation, and water. They are usually found in fecally-polluted water and are often associated with disease outbreaks. Although they arenot usually pathogenic themselves, their presence in drinking water indicates the possible presence of pathogens. E. coli, one species of the coliform group, is always found in feces and is, therefore, a more direct indicator of fecal contamination and the possible presence of enteric pathogens. |
Notes |
EPA 821-R-02-024. September 2002. Cover title. Includes bibliographical references. |
Contents Notes |
Scope and application -- Summary of method -- Definitions -- Interferences and contamination -- Safety -- Equipment and supplies -- Reagents and standards -- Sample collection, preservation, and storage -- Calibration and standardization -- Quality control (QC) -- Procedure -- Data analysis and calculations -- Method performance -- Pollution prevention -- Waste management -- References -- Tables and figures. |
Place Published |
Washington, DC |
Availability Notes |
Product reproduced from digital image. Order this product from NTIS by: phone at 1-800-553-NTIS (U.S. customers); (703)605-6000 (other countries); fax at (703)605-6900; and email at orders@ntis.gov. NTIS is located at 5285 Port Royal Road, Springfield, VA, 22161, USA. |
Corporate Au Added Ent |
United States. Environmental Protection Agency. Office of Water. |
Alternate Title |
Method 1604. |
PUB Date Free Form |
2002 |
BIB Level |
m |
Medium |
unmediated |
Content |
text |
Carrier |
volume |
Cataloging Source |
RDA |
OCLC Time Stamp |
20150929085307 |
Language |
eng |
Origin |
OCLC |
Type |
MERGE |
OCLC Rec Leader |
01622nam 2200337Ki 45010 |