Main Title |
Method 1603 : Escherichia coli (E. coli) in water by membrane filtration using modified membrane-thermotolerant Escherichia coli agar (modified mTEC). |
CORP Author |
Environmental Protection Agency, Washington, DC. Office of Water. |
Publisher |
United States, Environmental Protection Agency, Office of Water, |
Year Published |
2002 |
Report Number |
EPA/821/R-02/023 |
Stock Number |
PB2003-100128 |
Subjects |
Escherichia coli--United States--Prevention
|
Additional Subjects |
Escherichia coli ;
Membrane filtration ;
Water sampling ;
Bacteria ;
Agar ;
Epidemiology ;
Water pollution effects ;
Marine environment ;
Fresh water ;
Health effects ;
Gastrointestines ;
Feces ;
Substrates ;
Surface waters ;
Water quality ;
Counting methods ;
Calibration ;
Contamination ;
Laboratories ;
Test methods
|
Internet Access |
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB2003-100128 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Abstract |
The method describes a revised Escherichia coli membrane filter (MF) procedure, a single-step method that uses one medium, modified mTEC Agar, and does not require the transfer of the membrane filter to another medium or other substrate. The modified medium contains a chromogen (5-bromo-6-chloro- 3-indolyl--D-glucuronide), which is catabolized to glucuronic acid and a red- or magenta-colored compound byE. coli that produce the enzyme D-glucuronidase. The apparatus and equipment, and sampling, filtration, and verification procedures for this modified mTEC method are identical to those of the original mTEC method. Because the bacterium is a natural inhabitant only of the intestinal tract of warm-blooded animals, its presence in water samples is an indication of fecal pollution and the possible presence of entericpathogens. |
Notes |
"September 2002." "EPA-821-R-02-023." Includes bibliographical references. Microfiche. |
Place Published |
{Washington, D.C.} : |
Availability Notes |
Product reproduced from digital image. Order this product from NTIS by: phone at 1-800-553-NTIS (U.S. customers); (703)605-6000 (other countries); fax at (703)605-6900; and email at orders@ntis.gov. NTIS is located at 5285 Port Royal Road, Springfield, VA, 22161, USA. |
Corporate Au Added Ent |
United States. Environmental Protection Agency. Office of Water. |
PUB Date Free Form |
{2002} |
NTIS Prices |
PC A03/MF A01 |
BIB Level |
m |
Cataloging Source |
OCLC/T |
OCLC Time Stamp |
20050119131652 |
Language |
eng |
Origin |
NTIS |
Type |
MERGE |
OCLC Rec Leader |
00968nam 2200241Ka 45020 |