| Main Title |
Tissue Cell Culture Studies Comparative Cytotoxicity of Catalyst M and Catalyst I, with Cover Letter dated 04/20/1994. |
| CORP Author |
Dow Corning Corp., Midland, MI.; Environmental Protection Agency, Washington, DC. Office of Toxic Substances. |
| Year Published |
1994 |
| Report Number |
OTS-86940001474 |
| Stock Number |
OTS0558183 |
| Additional Subjects |
Toxicology ;
Health effects ;
Toxic substances ;
Siloxanes ;
Silicones ;
Di-Me ;
Laboratory animals ;
Acute toxicity ;
Mammals ;
Humans ;
In vitro ;
CAS No 301-10-0 ;
CAS No 63148-62-9
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
OTS0558183 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
40p |
| Abstract |
The tissue cell culture tests are designed to determine the cytopathic effects of a material or its extracts in contact with monclayers of diploid human cells. Since both test materials are liquids, each was applied to sterile filter pads for evaluation. The catalysts were tested for direct contact cytotoxicity by placing treated filter pads on confluent monolayers of human embryonic or fetal cells. After incubation for 24 hours, the cytopathic effects were evaluated microscopically against both a positive and a negative control. In addition, each test material (on filter pads) was extracted in dimethyl sulfoxide (DMSO) for one hour at 121C and in tissue culture medium (MEM) at 37C for 24 hours. The MER extracts were tested by aspirating the medium from acceptable wells and replacing it vich 1.0 - 1.5 al of the MEM extract from the test material. This was repeated using blank (no material) and positive control extracts. Test and control DMSD extracts were evaluated by replacing the medium on confluent monolayers vich extracts diluted to 21 in MEM. All cest wells were incubated and evaluated using the procedures described above for the direct rentact method. Both catalysts were cytopathic in direct contact, and their MEN extracts also caused morphological changes. While DMSO extracts of Catalyst 1 produced cytopathic effects in culture, those of Catalyst N appeared to be innocuous at the concentration tested. Extraction of Catalyst M at autoclave temperatures produced a large amount of yellow precipitate; as judged by the color change of phenol red in the medium, NEM extracts of this material vere quite acidic. |
| Supplementary Notes |
The quality of the documents listed in the Office of Toxic Substances database may not meet usual NTIS standards but are included to further the opportunity for the scientific and technical community to locate materials which may not be available elsewhere. The content is the responsibility of OTS. Sponsored by Environmental Protection Agency, Washington, DC. Office of Toxic Substances. |
| Availability Notes |
Product reproduced from digital image. Order this product from NTIS by: phone at 1-800-553-NTIS (U.S. customers); (703)605-6000 (other countries); fax at (703)605-6900; and email at orders@ntis.gov. NTIS is located at 5285 Port Royal Road, Springfield, VA, 22161, USA. |
| PUB Date Free Form |
28 Apr 1994 |
| Category Codes |
57Y; 57U; 68G; 99; 44G |
| NTIS Prices |
PC A04/MF A04 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
010500880 |
| Origin |
NTIS |
| Type |
CAT |
