||Analysis of DNA Strand Breaks Induced in Rodent Liver In vivo, Hepatocytes in Primary Culture, and a Human Cell Line by Chlorinated Acetic Acids and Chlorinated Acetaldehydes.
Chang, L. W. ;
Daniel, F. B. ;
DeAngelo, A. B. ;
||Health Effects Research Lab., Research Triangle Park, NC.
DNA damage ;
Acetic acids ;
Cultured cells ;
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||An alkaline unwinding assay was used to quantitate the induction of DNA strand breaks (DNA SB) in the livers of rats and mice treated in vivo, in rodent hepatocytes in primary culture, and in CCRF-CEM cells, a human lymphoblastic leukemia cell line, following treatment with tri- (TCA), di- (DCA), and mono- (MCA) chloroacetic acid and their corresponding aldehydes, tri- (chloral hydrate, CH), di- (DCAA) and mono- (CAA) chloroacetaldehyde. The continuous exposure of mice to 5 g/L DCA in the drinking water for 7 and 14 days did not induce appreciable hepatic DNA SB (< 10% at 14 days), although peroxisome proliferation, as evidenced by an increased cyanide-insensitive palmitoyl CoA oxidase (PCO) activity, was stimulated to 490% (7 days) and 652% (14 days) of control. Under this protocol, DENA (0.1 g/L) produced DNA damage after both 7 days (73% of control) and 14 days (57% of control).
||Pub. in Environmental and Molecular Mutagenesis 20, n4 p277-288 Dec 92. See also PB92-164904.
|NTIS Title Notes
||Reprint: Analysis of DNA Strand Breaks Induced in Rodent Liver In vivo, Hepatocytes in Primary Culture, and a Human Cell Line by Chlorinated Acetic Acids and Chlorinated Acetaldehydes.
||PC A03/MF A01