Main Title |
Method 1103.1: Escherichia coli (E. coli) in Water by Membrane Filtration Using Membrane-Thermotolerant Escherichia coli Agar (mTEC), July 2006. |
CORP Author |
Environmental Protection Agency, Washington, DC. Office of Water. |
Publisher |
Jul 2006 |
Year Published |
2006 |
Report Number |
EPA/821/R-06/010; |
Stock Number |
PB2007-101966 |
Additional Subjects |
Escherichia coli ;
Membrane filtration ;
Water sampling ;
Bacteria ;
Agar ;
Enumeration ;
Epidemiology ;
Microorganisms ;
Criteria ;
Water quality ;
Marine environment ;
Health effects ;
Gastroenteristis ;
Feces ;
Water pollution effects ;
Surface waters ;
Counting methods ;
Calibration ;
Growth(Biology) ;
Contamination ;
Quality control ;
Pollution prevention ;
Laboratories ;
Test methods ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB2007-101966 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
45p |
Abstract |
The MF method provides a direct count of bacteria in water based on the development of colonies on the surface of the membrane filter (Reference 18.4). A water sample is filtered through the membrane which retains the bacteria. After filtration, the membrane containing the bacterial cells is placed on a selective and differential medium, mTEC, incubated at 35DGC for 2 h to resuscitate injured or stressed bacteria, and then incubated at 44.5DGC for 22 h. Following incubation, the filter is transferred to a filter pad saturated with urea substrate. After 15 min, yellow, yellow-green or yellow-brown colonies are counted with the aid of a fluorescent lamp and a magnifying lens. |
Supplementary Notes |
See also PB2005-110685. |
Availability Notes |
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Category Codes |
57K; 57U; 68D |
NTIS Prices |
PC A04 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
005100950 |
Origin |
NTIS |
Type |
CAT |