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RECORD NUMBER: 10 OF 142

OLS Field Name OLS Field Data
Main Title Biliary Lipids Support Serum-Free Growth of 'Giardia lamblia'.
Author Gillin, F. D. ; Gault, M. J. ; Hofmann, A. F. ; Gurantz, D. ; Sauch, J. F. ;
CORP Author California Univ. Medical Center, San Diego.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1986
Report Number EPA-R-811950; EPA/600/J-86/226;
Stock Number PB87-170437
Additional Subjects Giardia ; Parasitic diseases ; Small intestine diseases ; Lipids ; Blood serum ; Bile ; Growth ; Reprints ; Giardia lamblia ; Phosphatidylcholines ; Glycocholic acid ; Glycodeoxycholic acid
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NTIS  PB87-170437 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 7p
Abstract Giardia lamblia has only been grown in vitro in media containing serum or serum fractions. How this pathogen can grow in the human small intestinal lumen without serum is not known. The authors found that samples of human hepatic or gall bladder bile maintained G. lamblia survival, but not growth, for 24 to 48 hours, in medium without serum or protein. In contrast, an artificial biliary lipid (BL) dispersion containing six bile salts, phosphatidylcholine (PC), and cholesterol, in the ratios characteristic of human bile, supported parasite growth in serum and protein-free medium. To define the requirements, the authors showed that 1-palmitoyl, 2-linoleoyl-PC or 1-palmitoyl, 2-oleoyl-PC (which predominate in human bile) satisfied the requirement for PC. Moreover, either glycocholate or glycodeoxycholate could be substituted for the bile salt mixture. The finding that BL can support serum-free growth of G. lamblia may help explain why this parasite colonizes the upper small intestine. (Copyright (c) 1986, American Society for Microbiology.)
Supplementary Notes Pub. in Infection and Immunity, v53 n3 p641-645 Sep 86. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
NTIS Title Notes Journal article.
Title Annotations Reprint: Biliary Lipids Support Serum-Free Growth of 'Giardia lamblia'.
PUB Date Free Form c1986
Category Codes 57N; 57E
NTIS Prices PC A02/MF A01
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 716918592
Origin NTIS
Type CAT