Full Record Display for the EPA National Library Catalog

RECORD NUMBER: 1113 OF 1378

OLS Field Name OLS Field Data
Main Title Stable Carbon Isotope Analysis of Nucleic Acids to Trace Sources of Dissolved Substrates Used by Estuarine Bacteria.
Author Coffin, R. B. ; Velinsky, D. J. ; Devereux, R. ; Price, W. A. ; Cifuentes, L. A. ;
CORP Author Technical Resources, Inc., Gulf Breeze, FL. ;Carnegie Institution of Washington, DC. Geophysical Lab. ;Illinois Univ. at Urbana-Champaign. Dept. of Veterinary Pathobiology.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/388;
Stock Number PB91-164012
Additional Subjects Aquatic microbiology ; Tracer studies ; Organic matter ; Nucleic acids ; Isotopic labeling ; Estuaries ; Carbon isotopes ; Substrates ; Aquatic ecosystems ; Bioassay ; Deoxyribonucleic acids ; Mass spectroscopy ; Ribonucleic acids ; Extraction ; Microorganisms ; Bacteria ; Water pollution detection ; Environmental transport ; Reprints ;
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB91-164012 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/13/1991
Collation 11p
Abstract The natural abundance of stable carbon isotopes measured in bacterial nucleic acids that were extracted from estuarine bacterial concentrates were used to trace sources of organic matter for bacteria in aquatic environments. The stable carbon isotope ratios of P. aeruginosa and nucleic acids extracted from cultures resembled the carbon source on which bacteria were grown. The carbon isotope discrimination (delta) between substrate and bacterial cultures averages +2.3 + or - 0.6% (n=13). Generally the lack of isotope discrimination between bacteria and nucleic acids that was noted in the laboratory was observed in the field. Exceptions to this comparison were due to changes in bacterial substrate sources as a result of the incubation experiments that were used to obtain bacteria for isotope analysis. The authors results from work in the field and laboratory indicate that the approach is useful for tracing sources of and describing the bacterial role in cycling of dissolved organic matter in aquatic environments.
Supplementary Notes Pub. in Applied and Environmental Microbiology, v56 n7 p2012-2020 Jul 90. Prepared in cooperation with Carnegie Institution of Washington, DC. Geophysical Lab., and Illinois Univ. at Urbana-Champaign. Dept. of Veterinary Pathobiology. Sponsored by Environmental Research Lab., Gulf Breeze, FL.
NTIS Title Notes Journal article.
Title Annotations Reprint: Stable Carbon Isotope Analysis of Nucleic Acids to Trace Sources of Dissolved Substrates Used by Estuarine Bacteria.
Category Codes 47D; 57H; 57K; 68D; 99A
NTIS Prices PC A03/MF A01
Primary Description 600/04
Document Type NT
Cataloging Source NTIS/MT
Control Number 113027821
Origin NTIS
Type CAT