Main Title |
Fiber Optic Fluoroimmunosensor for the Detection of Polychlorinated Biphenyls. |
Author |
Rogers, K. R. ;
Van Emon, J. M. ;
Kline, R. ;
Wright, J. ;
Zhao, Q. ;
|
CORP Author |
Maryland Univ. at Baltimore.;Environmental Monitoring Systems Lab., Las Vegas, NV. |
Publisher |
Feb 95 |
Year Published |
1995 |
Report Number |
EPA-CR-820460; EPA/600/A-95/058; |
Stock Number |
PB95-218855 |
Additional Subjects |
Fluorometers ;
Chlorinated organic compounds ;
DNA adducts ;
Reprints ;
Fiber optics ;
Technology innovation ;
Analytical chemistry ;
Field tests ;
Optical measuring instruments ;
Fluorescence spectroscopy ;
Immunoassay ;
Pollution monitoring ;
Hazardous materials ;
Toxic substances ;
Fluoroimmunosensor ;
PCBs(Polychlorinated biphenyls) ;
Anti-PCB serum ;
Evanescent wave fluorometer
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB95-218855 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
10p |
Abstract |
There currently exists a critical and growing need for cost-effective, real-time, and in situ methods for detection of environmental pollutants such as polychlorinated biphenyls (PCBs). Herein reported is a evanescent wave Fluoroimmununosensor for measurement of PCBS. 2,4-5-Trichlorophenoxybutyric acid (TCPB) was cross linked to fluorescein ethyleneamine (TCPB-FL) and used as a optical signal generator. Protein A purified IgG, obtained from anti-PCB serum, was immobilized on quartz fibers and used as the biological recognition element in an evanescent wave fluorimeter. The optical signal was generated by the binding of the fluorescent probe TCPB-FL to the antibody (Ab)-coated fiber. Fluorescent probe concentrations in the nM range resulted in reversible binding. Several Aroclors displaced the fluorescent probe (TCPB-FL) in a concentration-dependent manner. Arochlor 1248 was detected from the ppb to ppm range. Arochlor 1242 was also measured in methanol extracts from standard reference material soils. |
Supplementary Notes |
Presented at the International Symposium on Field Screening Methods for Hazardous Wastes and Toxic Chemicals (4th), Las Vegas, NV., February 22-25, 1995. Sponsored by Environmental Monitoring Systems Lab., Las Vegas, NV. |
Highlights Notes |
%AUT:M. E. /Eldefrawi |
Title Annotations |
Reprint: Fiber Optic Fluoroimmunosensor for the Detection of Polychlorinated Biphenyls. |
Category Codes |
68G; 68; 99A; 57E; 57B; 57U |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/07 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
521404130 |
Origin |
NTIS |
Type |
CAT |