Main Title |
Methods Used in Determination of Cholinesterase Activity. |
Author |
Mineau., P. ;
|
CORP Author |
Corvallis Environmental Research Lab., OR. ;ManTech Environmental Technology International, Inc., Corvallis, OR. ;Clemson Univ., SC. Inst. of Wildlife and Environmental Toxicology. |
Publisher |
c1991 |
Year Published |
1991 |
Report Number |
EPA/600/A-92/078; |
Stock Number |
PB92-179035 |
Additional Subjects |
Cholinesterase ;
Toxicology ;
Cholinesterase inhibitors ;
Test methods ;
pH ;
Temperature ;
Cations ;
Substrate specificity ;
Wildlife ;
Reprints ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB92-179035 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
38p |
Abstract |
The chapter provides an overview and historical perspective of the many available methods for cholinesterase (ChE) activity determination. Due to the almost universal use of the spectrophotometric assay in wildlife toxicology, the remainder of the chapter focuses on this technique. Sources of variability in the assay are discussed, including assay temperature, instrumentation, degree of solubilization of membrane-bound enzyme, and lipid concentration in serum samples. Methods to control these sources of error are offered. Consideration is given to methods of differentiating the activity of the various esterases (for example, acetylcholinesterase and butyrylcholinesterase). A brief review is provided of commercial kits available for field and/or laboratory testing of samples for ChE activity. The chapter includes a discussion of future innovations and refinements of the ChE assay, including the use of reactivation techniques and adaptation of the assay for use with 96-well microtitration plates. (Copyright (c) 1991 Elsevier Science Publishers B.V.) |
Supplementary Notes |
Pub. in Cholinesterase-Inhibiting Insecticides, 1991. See also PB88-219282. Prepared in cooperation with ManTech Environmental Technology International, Inc., Corvallis, OR., and Clemson Univ., SC. Inst. of Wildlife and Environmental Toxicology. |
NTIS Title Notes |
Book chapter. |
Title Annotations |
Reprint: Methods Used in Determination of Cholinesterase Activity. |
Category Codes |
57F; 57B; 57Y; 48B |
NTIS Prices |
PC A03/MF A01 |
Primary Description |
600/02 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
217729992 |
Origin |
NTIS |
Type |
CAT |