Main Title |
Ring Fission of Anthracene by a Eukaryota. |
Author |
Hammel, K. E. ;
Green, B. ;
Gai, W. Z. ;
|
CORP Author |
State Univ. of New York Coll. of Environmental Science and Forestry, Syracuse. Dept. of Chemistry.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. |
Publisher |
c1993 |
Year Published |
1993 |
Report Number |
EPA-R-813530; EPA/600/J-93/191; |
Stock Number |
PB93-199503 |
Additional Subjects |
Aromatic polycyclic hydrocarbons ;
Pollutants ;
Biodeterioration ;
Anthracene ;
Lignin ;
Land pollution ;
Air pollution ;
Sediments ;
Fungi ;
Biochemistry ;
Oxidation ;
In vivo analysis ;
Carbon dioxide ;
Reprints ;
Ring fission ;
Phanerochaete chrysosporium ;
Chemical reaction mechanisms
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB93-199503 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
6p |
Abstract |
Ligninolytic fungi are unique among eukaryotes in their ability to degrade polycyclic aromatic hydrocarbons (PAHs), but the mechanism for this process is unknown. Although certain PAHs are oxidized in vitro by the fungal lignin peroxidases (LiPs) that catalyze ligninolysis, it has never been shown that LiPs initiate PAH degradation in vivo. To address these problems, the metabolism of anthracene (AC) and its in vitro oxidation product, 9,10-anthraquinone (AQ), was examined by chromatographic and isotope dilution techniques in Phanerochaete chrysosporium. The fungal oxidation of AC to AQ was rapid, and both AC and AQ were significantly mineralized. Both compounds were cleaved by the fungus to give the same ring-fission metabolite, phthalic acid, and phthalate production from AQ was shown to occur only under ligninolytic culture conditions. These results show that the major pathway for AC degradation in Phanerochaete proceeds AC -> AQ -> phthalate + CO2 and that it is probably mediated by LiPs and other enzymes of ligninolytic metabolism. |
Supplementary Notes |
Pub. in Proceedings of National Academy of Science, v88 p10605-10608 Dec 91. Sponsored by Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Ring Fission of Anthracene by a Eukaryota. |
Category Codes |
68; 68A; 57K; 57B; 99F |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/14 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
321821702 |
Origin |
NTIS |
Type |
CAT |