Main Title |
Assay for Detection and Enumeration of Genetically Engineered Microorganisms Which Is Based on the Activity of a Deregulated 2,4-Dichlorophenoxyacetate Monooxygenase. |
Author |
King, R. J. ;
Short, K. A. ;
Seidler, R. J. ;
|
CORP Author |
Corvallis Environmental Research Lab., OR. ;NSI Technology Services Corp., Corvallis, OR. Environmental Research Lab. |
Publisher |
cJun 91 |
Year Published |
1991 |
Report Number |
EPA/600/J-94/469; |
Stock Number |
PB95-137311 |
Additional Subjects |
Assaying ;
Genetics ;
Microorganisms ;
Chemical analysis ;
Biotechnology ;
Phenol ;
Cells(Biology) ;
Cell cultures ;
Oxygenases ;
Chromatographic analysis ;
Reprints ;
Oxygenase/2-4-Dichlorophenoxyacetate ;
Genetic engineering ;
Pseudomonas aeruginosa ;
Pseudomonas putida
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB95-137311 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
5p |
Abstract |
An assay system was developed for the enumeration of genetically engineered microorganisms expressing a deregulated 2,4-dichlorophenoxyacetate (TFD) monooxygenase, which coverts phenoxyacetate (PAA) to phenol. In PAA-amended cultures of Pseudomonas aeruginosa PAO1C(pRO103) and Pseudomonas putida PPO301(pRO103), strains which express a deregulated TFD monoxygenase, phenol production was proprtional to cell number. Phenol was reacted, under specific conditions, with a 4-aminoantipyrine dye to form an intensely colored dye-phenol complex (AAPPC), which when measured spectrophotmetrically could detect as few as 10(3) cells per ml. The assay was corrobrated by monitoring the disappearnace of PAA and the accumulation of phenol by high-performance liquid chromatography and gas chromatography. |
Supplementary Notes |
Pub. in Applied and Environmental Microbiology, v57 n6 p1790-1792 Jun 91. Prepared in cooperation with NSI Technology Services Corp., Corvallis, OR. Environmental Research Lab. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Assay for Detection and Enumeration of Genetically Engineered Microorganisms Which Is Based on the Activity of a Deregulated 2,4-Dichlorophenoxyacetate Monooxygenase. |
Category Codes |
57K; 57F |
NTIS Prices |
PC A01/MF A01 |
Primary Description |
600/02 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
500622169 |
Origin |
NTIS |
Type |
CAT |