Full Record Display for the EPA National Library Catalog

RECORD NUMBER: 131 OF 427

OLS Field Name OLS Field Data
Main Title Evaluation of Sucrose as an Alternative to Sodium Chloride in the Microtox (Trade Name) Assay: Comparison to Fish and Cladoceran Tests with Freshwater Effluents.
Author Ankley, G. T. ; Peterson, G. S. ; Amato, J. R. ; Jenson, J. J. ;
CORP Author Environmental Research Lab.-Duluth, MN. ;AScI Corp., Duluth, MN.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/210;
Stock Number PB91-144972
Additional Subjects Aquatic biology ; Mutagenicity tests ; Sucrose ; Sodium chloride ; Toxic substances ; Water pollution effects(Animals) ; Industrial effluents ; Reprints ; Microtox assay ; Pimephales promelas ; Ceriodaphnia dubia
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB91-144972 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/13/1991
Collation 8p
Abstract The toxicity of 44 freshwater effluents was evaluated using the fathead minnow (Pimephales promelas), a cladoceran (Ceriodaphnia dubia) and Microtox (Photobacterium phosphoreum). The latter assay was conducted with both sodium chloride (NaCl) and sucrose for osmotic protection of the bacteria in an effort to determine whether the use of sucrose increased the sensitivity of Microtox to samples exhibiting toxicity to the fish and cladoceran species. Twenty-three of the effluents tested were toxic to fathead minnows and/or C. dubia; of these, Microtox was sensitive to 10 when tested in NaCl and seven when tested in sucrose. In one instance testing in sucrose predicted toxicity to the fish and cladoceran species when testing in NaCl did not. Single chemical tests demonstrated that Microtox sensitivity was greater to zinc chloride, nickel chloride and methanol when tested in sucrose as opposed to NaCl, about equal to copper sulfate and phenol in sucrose and NaCl, and was far greater to chlorine when tested in NaCl than in sucrose. These results, as well as data from Microtox assays with effluents containing known toxicants, suggest that the use of sucrose, in conjunction with NaCl, for osmotic adjustment in the assay could be helpful as part of a suite of tests for identifying general classes of compounds responsible for toxicity in freshwater effluents.
Supplementary Notes Pub. in Environmental Toxicology and Chemistry, v9 p1305-1310 1990. Prepared in cooperation with AScI Corp., Duluth, MN.
NTIS Title Notes Journal article.
Title Annotations Reprint: Evaluation of Sucrose as an Alternative to Sodium Chloride in the Microtox (Trade Name) Assay: Comparison to Fish and Cladoceran Tests with Freshwater Effluents.
Category Codes 57Y; 57F; 68D
NTIS Prices PC A02/MF A01
Primary Description 600/03
Document Type NT
Cataloging Source NTIS/MT
Control Number 109129960
Origin NTIS
Type CAT