||Vibrio cholerae 01 Can Assume a 'Rugose' Survival Form That Resists Killing by Chlorine, Yet Retains Virulence.
Rice, E. W. ;
Johnson, C. H. ;
Clark, R. M. ;
Fox, K. R. ;
Reasoner, D. J. ;
||Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. ;Maryland Univ. at Baltimore. School of Medicine.;Department of Veterans Affairs Medical Center, Baltimore, MD.;Public Health Service, Rockville, MD.
Vibrio cholerae ;
Cultured cells ;
Microbial colony count ;
Microbial sensitivity tests ;
Bacterial adhesion ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||Vibrio cholerae 01 is able to shift between smooth and rugose colonial morphologies. Cultures of smooth V. cholerae strains were inactivated in less than 20 s at a concentration of 1.0 mg/l free chlorine. In contrast, cultures of rugose variants exposed to this concentration of chlorine showed an initial rapid drop in viable counts, followed by persistence of a protected subpopulation of cells. Viable V. cholerae could still be recovered from rugose cultures even after exposure to 2.0 mg/l free chlorine for 30 min. Preliminary studies suggest that resistance to killing by chlorine was due to formation of cell aggregates enclosed in a gelatinous mucoid material. Rugose strains appeared to be fully virulent, based on their ability to adhere to Caco-2 cells and elicit fluid accumulation in rabbit ileal loops. Our data suggest that the V. cholerae rugose phenotype represents a fully virulent survival form of the organism that can persist in the presence of free chlorine. (Copyright (c) 1993 Chapman & Hall.)
||Pub. in Jnl. of Health Research, v3 p89-98 1993. Prepared in cooperation with Maryland Univ. at Baltimore. School of Medicine. Sponsored by Department of Veterans Affairs Medical Center, Baltimore, MD., and Public Health Service, Rockville, MD.
|NTIS Title Notes
||Reprint: Vibrio cholerae 01 Can Assume a 'Rugose' Survival Form That Resists Killing by Chlorine, Yet Retains Virulence.
||PC A02/MF A01