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OLS Field Name OLS Field Data
Main Title Vibrio cholerae 01 Can Assume a 'Rugose' Survival Form That Resists Killing by Chlorine, Yet Retains Virulence.
Author Rice, E. W. ; Johnson, C. H. ; Clark, R. M. ; Fox, K. R. ; Reasoner, D. J. ;
CORP Author Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. ;Maryland Univ. at Baltimore. School of Medicine.;Department of Veterans Affairs Medical Center, Baltimore, MD.;Public Health Service, Rockville, MD.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-93/381; PHS-R01A128856;
Stock Number PB94-117504
Additional Subjects Vibrio cholerae ; Chlorine ; Virulence ; Cultured cells ; Phenotype ; Microbial colony count ; Microbial sensitivity tests ; Rabbits ; Bacterial adhesion ; pH ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB94-117504 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 02/27/1994
Collation 10p
Abstract Vibrio cholerae 01 is able to shift between smooth and rugose colonial morphologies. Cultures of smooth V. cholerae strains were inactivated in less than 20 s at a concentration of 1.0 mg/l free chlorine. In contrast, cultures of rugose variants exposed to this concentration of chlorine showed an initial rapid drop in viable counts, followed by persistence of a protected subpopulation of cells. Viable V. cholerae could still be recovered from rugose cultures even after exposure to 2.0 mg/l free chlorine for 30 min. Preliminary studies suggest that resistance to killing by chlorine was due to formation of cell aggregates enclosed in a gelatinous mucoid material. Rugose strains appeared to be fully virulent, based on their ability to adhere to Caco-2 cells and elicit fluid accumulation in rabbit ileal loops. Our data suggest that the V. cholerae rugose phenotype represents a fully virulent survival form of the organism that can persist in the presence of free chlorine. (Copyright (c) 1993 Chapman & Hall.)
Supplementary Notes Pub. in Jnl. of Health Research, v3 p89-98 1993. Prepared in cooperation with Maryland Univ. at Baltimore. School of Medicine. Sponsored by Department of Veterans Affairs Medical Center, Baltimore, MD., and Public Health Service, Rockville, MD.
NTIS Title Notes Journal article.
Title Annotations Reprint: Vibrio cholerae 01 Can Assume a 'Rugose' Survival Form That Resists Killing by Chlorine, Yet Retains Virulence.
Category Codes 57K; 57Y
NTIS Prices PC A02/MF A01
Primary Description 600/14
Document Type NT
Cataloging Source NTIS/MT
Control Number 400732864
Origin NTIS
Type CAT