Main Title |
Efficiency of Human Rotavirus Propagation in Cell Culture. |
Author |
Ward, R. L. ;
Knowlton, D. R. ;
Pierce, M. J. ;
|
CORP Author |
Christ Hospital, Cincinnati, OH. Inst. of Medical Research.;Health Effects Research Lab., Research Triangle Park, NC. |
Year Published |
1984 |
Report Number |
EPA-R-810341; EPA/600/J-84/373; |
Stock Number |
PB86-201464 |
Additional Subjects |
Cell cultures ;
Rotaviruses ;
Efficiency ;
Viruses ;
Humans ;
Growth ;
Monkeys ;
Reprints ;
Cell lines
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB86-201464 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
10p |
Abstract |
The study was designed to find methods to reproducibly propagate human rotaviruses from fecal specimens and to determine the relationship between particle numbers and infectivity. Growth of virus was initially compared in primary and continuous lines of monkey kidney cells. Primary cells (African green and cynomolgus monkey kidney) supported virus growth directly from fecal specimens much more efficiently than continuous lines of African Green (CV-1) or rhesus (MA104) monkey kidney cells. Rotaviruses were grown in primary cells from 14/14 fecal specimens of different individuals collected over a three-year period. Although rotaviruses in fecal samples could not always be grown in the continuous cell lines, two passages in primary cells appeared to fully adapt the viruses for propagation in the continuous cell line tested (MA104). The efficiency of rotavirus growth was quantified with five of the fecal isolates. It was calculated that, on the average, 1 out of every 46,000 passages in primary cells, an average of 1 out of every 6,600 progeny virus particles appeared to be infectious. Thus, rotaviruses in fecal specimens were consistently grown in primary cells and passage in these cells both increased virus infectivity and adapted the viruses for growth in continuous cell lines. |
Supplementary Notes |
Pub. in Jnl. of Clinical Microbiology, v19 n6 p748-753 Jun 84. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Efficiency of Human Rotavirus Propagation in Cell Culture. |
PUB Date Free Form |
c1984 |
Category Codes |
6M; 57K |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
621322870 |
Origin |
NTIS |
Type |
CAT |