Main Title |
Cytotoxicity of Chemical Carcinogens Towards Human Bronchial Epithelial Cells Evaluated in a Clonal Assay. |
Author |
Siegfried, J. M. ;
Nesnow, S. ;
|
CORP Author |
Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. |
Publisher |
c1984 |
Year Published |
1984 |
Report Number |
EPA-68-02-4031; EPA/600/J-84/412; |
Stock Number |
PB89-103618 |
Additional Subjects |
Toxicology ;
Carcinogens ;
Bronchii ;
Clone cells ;
Toxicity ;
Cell lines ;
Epithelium ;
Culture media ;
Methodology ;
Reprints ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB89-103618 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
7p |
Abstract |
Survival of human bronchial epithelial cells after administration of four chemical carcinogens was measured in a clonal assay. Human bronchial epithelial cells were obtained from outgrowths of explanted tissue pieces. Serum-free medium was used for both explant culture and clonal growth. The clonal assay could be performed on three substrata: plastic dishes alone, protein-coated dishes, and inactivated Swiss 3T3 cells. Several other cell lines supported clonal growth of the human cells. Little variation among individuals in cytotoxicity of N-methyl-N'-nitro-N-nitrosoguanidine and K2CrO4, a possible human lung carcinogen, was observed, but in the case of benzo(a)pyrene and 7,12-dimethylbenz(a)anthracene, large variations in survival were found between cultures derived from different individuals. (Copyright (c) IRL Press Ltd.) |
Supplementary Notes |
Pub. in Carcinogenesis, v5 p1317-1322 1984. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Cytotoxicity of Chemical Carcinogens Towards Human Bronchial Epithelial Cells Evaluated in a Clonal Assay. |
Category Codes |
57Y; 57F |
NTIS Prices |
Not available NTIS |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
833026246 |
Origin |
NTIS |
Type |
CAT |