Main Title |
Induction of Mutations by Chemical Agents at the Hypoxanthine-Guanine Phosphoribosyl Transferase Locus in Human Epithelial Teratoma Cells. |
Author |
Huberman, E. ;
McKeown, C. K. ;
Jones, C. A. ;
Hoffman, D. R. ;
Murao, S. ;
|
CORP Author |
Argonne National Lab., IL. ;Oak Ridge National Lab., TN.;Health Effects Research Lab., Research Triangle Park, NC. |
Year Published |
1984 |
Report Number |
EPA/600/J-84/357; |
Stock Number |
PB86-162237 |
Additional Subjects |
Toxicology ;
Humans ;
Cells(Biology) ;
Chromosomes ;
Reprints ;
Mutagens ;
Thioguanine ;
Cell lines
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB86-162237 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
13p |
Abstract |
Induction of 6-thioguanine (TG) resistance by chemical mutagens was examined in a line of cells derived from a human epithelial teratocarcinoma cell clone. The cells, designated as P3 cells, have a stable diploid karyotype with 46(XX) chromosomes, including a translocation between chromosomes 15 and 20. Efficient recovery of TG-resistant mutants induced by the direct-acting mutagens: N-methyl-N'-nitro-N-nitrosoguanidine (MNNG); 7beta,8alpha-dihydroxy-9alpha,10alpha-epoxy-7,8,9,10-tetrahydrobenzo(alpha)pyrene (BPDE); and benzo(alpha)pyrene (B(alpha)P); activated in a cell-mediated assay, required an expression time of 7 days and a saturation density of 2X10 to the fourth power cells/60-mm petri dish. The TG-resistant mutant cells induced by MNNG and BPDE maintained their resistant phenotype 4-6 weeks after isolation. This mutant phenotype was associated with a morre than 10-fold reduction in hypoxanthine-guanine phosphoribosyl transferase (HGPRT) activity relative to that of the parental P3 cell line, which was shown to catalyze the formation of 4.6 pmoles inosine-5'-monophosphate (IMP)/min/micrograms protein. Induction of TG resistance was also observed in P3 cells cocultivated in a cell-mediated assay with human breast carcinoma cells. Which are capable of polycyclic aromatic hydrocarbon (PAH) metabolism. |
Supplementary Notes |
Pub. in Mutation Research 130, p127-137 1984. Prepared in cooperation with Oak Ridge National Lab., TN. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Induction of Mutations by Chemical Agents at the Hypoxanthine-Guanine Phosphoribosyl Transferase Locus in Human Epithelial Teratoma Cells. |
PUB Date Free Form |
c1984 |
Category Codes |
6T; 57Y; 57F |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
612915168 |
Origin |
NTIS |
Type |
CAT |