Main Title |
Determination of 'Giardia lamblia' Cyst Infective Dose for the Mongolian Gerbil ('Meriones unguiculatus'). |
Author |
Schaefer, F. W. ;
Johnson, C. H. ;
Hsu, C. H. ;
Rice., E. W. ;
|
CORP Author |
Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. ;Computer Sciences Corp., Cincinnati, OH. |
Publisher |
c1991 |
Year Published |
1991 |
Report Number |
EPA/600/J-91/171; |
Stock Number |
PB91-231399 |
Additional Subjects |
Water quality ;
Giardia lamblia ;
Potable water ;
Virulence ;
Gerbils ;
Dose-response relationships ;
Regression analysis ;
Animal disease models ;
Reprints ;
Meriones unguiculatus
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB91-231399 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
4p |
Abstract |
The purpose of the study was to determine the I.D.(50) for Giardia lamblia (CDC:0284:1) cysts in Mongolian gerbils (Meriones unguiculatus) and compare it to human infectivity data. Cysts were purified from Mongolian gerbil feces and diluted to produce inocula for each dosage group. After observation of the gerbils for production of either cysts in the feces or trophozoites in the small intestine over a two week period, the I.D.(50) results calucated by Probit anaysis, Spearman-Karber, and Reed-Muench were 282, 288, and 219 cysts, respectively. A least squares regression analysis, relating the number of cysts inoculated and the percent of infectivity, estimated the log(10) I.D.(50) to be 2.50, with a coefficient of correlation (r) of 0.9689. The log(10) I.D.(50) derived by this method is similar to those calculated by the previous methods. Since human feeding studies indicate the I.D.(50) for humans is < or = 10 and the authors results indicate the I.D.(50) for gerbils to be around 285 cysts, the gerbil model can not be trusted to reflect what might happen in human exposures. |
Supplementary Notes |
Pub. in Applied and Environmental Microbiology, v57 n8 p2408-2409 Aug 91. Prepared in cooperation with Computer Sciences Corp., Cincinnati, OH. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Determination of 'Giardia lamblia' Cyst Infective Dose for the Mongolian Gerbil ('Meriones unguiculatus'). |
Category Codes |
57U; 57K |
NTIS Prices |
PC A01/MF A01 |
Primary Description |
600/14 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
128229647 |
Origin |
NTIS |
Type |
CAT |